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首页> 外文期刊>RSC Advances >Field-actuated antineoplastic potential of smart and versatile PEO-bPEI electrospun scaffold by multi-staged targeted co-delivery of magnetite nanoparticles and niclosamide-bPEI complexes
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Field-actuated antineoplastic potential of smart and versatile PEO-bPEI electrospun scaffold by multi-staged targeted co-delivery of magnetite nanoparticles and niclosamide-bPEI complexes

机译：磁铁矿纳米颗粒的多分阶段靶向共递送磁铁和多功能PEO-BPEI Electrome Electropul支架的现场致动抗肿瘤潜力和Niclosamide-BPEI复合物

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摘要

Surgical removal of tumor mass is often followed by its recurrence at the same or a distant location by proliferation and metastasis of remnant cancer cells at the site of surgery. In order to circumvent such complications, we have designed a biocompatible smart stimuli responsive anti-cancer nanofiber patch which can effectively eliminate cancer cells and, at the same time, acts as physical barrier for cancer metastasis. In pursuit of this, in this work we fabricated a composite bPEI-PEO nanofiber scaffold loaded with folic acid functionalized octagonal magnetite nanoparticles (FA-bPEI@Fe3O4 NPs) and bPEI-niclosamide complexes and further evaluated its field responsive anticancer therapeutic efficacy. Highly magnetic FA-bPEI@Fe3O4 NPs impregnated within nanofiber renders them susceptible to partial disintegration by an external alternating magnetic field (AMF) and thereby enables them to attain on-demand stipulated release of FA-bPEI@Fe3O4 NPs and bPEI-niclosamide complexes. Upon release, free FA-bPEI@Fe3O4 NPs and niclosamide perpetrated cell death by instigating apoptosis and oxidative stress (in KB cells (FR+) and L132 cells (FR-)) which was subsequently investigated on a qualitative and quantitative basis in a time dependent manner by fluorescence microscopy and flow cytometry, and further corroborated with semi-quantitative RT-PCR analysis. In summary, the composite nanofiber fabricated herein can manifest stipulated on-demand release of diverse bioactive molecules solely under the influence of external AMF and thereby effectively attain the therapeutic perquisites of cancer prognosis exactly where and when needed.
机译：手术去除肿瘤肿块通常在手术部位的胃肠癌细胞的增殖和转移时进行肿瘤肿块的再现或远离位置。为了规避此类并发症，我们设计了一种生物相容性智能刺激抗癌纳米纤维蛋白，可以有效地消除癌细胞，同时充当癌症转移的物理障碍。在追求这一点，在这项工作中，我们制造了装载的复合BPEI-PEO纳米纤维支架，其装有叶酸官能化八角形磁铁矿纳米粒子（FA-BPEI @ Fe3O4 NPS）和Bpei-Niclosamide络合物，并进一步评估其抗抗抗癌治疗效果。高磁性Fa-BPEI @ Fe3O4 NPS在纳米纤维内浸渍，它们易受外部交流磁场（AMF）部分崩解的影响，从而使它们能够实现FA-BPEI @ Fe3O4 NPS和Bpei-NiClosamide络合物的按需规定释放。在释放后，通过施加细胞凋亡和氧化应激（在KB细胞（FR +）和L132细胞（FR-）中，在随后在依赖于定性和定量的基础进行调查通过荧光显微镜和流式细胞术的方式，并进一步用半定量RT-PCR分析进行证实。总之，本文制造的复合纳米纤维可仅在外部AMF的影响下表现出各种生物活性分子的规定的按需释放，从而有效地达到癌症预后的治疗性腐败性，确切地在何处和时。

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  • 来源
    《RSC Advances》 |2016年第52期|共16页
  • 作者

    Sukumar Uday Kumar; Gopinath P.;

  • 作者单位

    Indian Inst Technol Roorkee Ctr Nanotechnol Nanobiotechnol Lab Roorkee 247667 Uttarakhand India;

    Indian Inst Technol Roorkee Ctr Nanotechnol Nanobiotechnol Lab Roorkee 247667 Uttarakhand India;

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  • 正文语种 eng
  • 中图分类 化学;
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