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Resveratrol-ZnO nanohybrid enhanced anti-cancerous effect in ovarian cancer cells through ROS

机译：白藜芦醇 - ZnO纳米嗜含量通过ROS增强卵巢癌细胞中的抗癌变效应

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The use of nanotechnology in medicine and more specifically in drug delivery is expected to spread rapidly. Currently many substances are under investigation for drug delivery and more specifically for cancer therapy. Nano-conjugation of the drug is likely to provide protection against degradation, increasing bioavailability, and improvement in intracellular penetration, enhanced efficacy and control delivery of the drug. In this study, ZnO nanoparticles (NPs) conjugated with a well-known anti-proliferative and chemopreventive trans-resveratrol (RSV) has been designed, characterized and found to be a potential drug for ovarian cancer treatment. Nano-conjugate (RSV-ZnO) has been characterized by FTIR, Raman scattering and Transmission electron microscopy (TEM). Picosecond-resolved fluorescence studies of RSV-ZnO nano-conjugate reveal efficient electron migration from ZnO NPs to RSV, eventually enhancing the ROS activity compared to free RSV. Various in vivo and in vitro studies including MTT assay and apoptosis studies on ovarian cancer (PA1) cell lines reveals the RSV-ZnO nano-conjugate to be more effective in cancer cell death in comparison to free RSV. DCFH assay (in vitro) and DCFDA method (in vivo in PA1 cell lines) demonstrate the huge enhancement of antioxidant property (through ROS) in case of nano-conjugate. JC-1 staining method unravels the increase in depolarization of the mitochondrial membrane in the PA1 cell upon nano-conjugate, consistent with mitochondrial dysfunction. Finally we have performed a Western blot study by expression of some proteins like actin, Bax, Bcl-2 and Caspase-9 to confirm apoptosis.
机译：预计使用纳米技术在药物中使用纳米技术和更具体地在药物递送中迅速传播。目前许多物质都在调查药物递送，更具体地用于癌症治疗。该药物的纳米缀合可能提供防止降解，增加生物利用度，提高药物的细胞内渗透，增强疗效和对照递送药物。在本研究中，设计了ZnO纳米颗粒（NPS）与众所周知的抗增殖和化学预防反式翻白剂（RSV）共轭，特征，并发现是卵巢癌治疗的潜在药物。纳米缀合物（RSV-ZnO）的特征在于FTIR，拉曼散射和透射电子显微镜（TEM）。 RSV-ZnO纳米缀合物的PICOSecond-DEARATVATVED荧光研究揭示了从ZnO NPS到RSV的有效电子迁移，最终增强了与游离RSV相比的ROS活性。体内和体外研究中的各种研究包括MTT测定和对卵巢癌（PA1）细胞系的凋亡研究揭示了RSV-ZnO纳米缀合物，与游离RSV相比，癌细胞死亡更有效。 DCFH测定（体外）和DCFDA方法（在PA1细胞系中的体内）证明了在纳米缀合物的情况下抗氧化特性（通过ROS）的巨大增强。 JC-1染色方法将PA1细胞在纳米缀合物上的线粒体膜的升高释放，与线粒体功能障碍一致。最后，我们通过表达肌动蛋白，BAX，BCL-2和Caspase-9等一些蛋白质进行了蛋白质印迹研究，以确认细胞凋亡。

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  • 来源
    《RSC Advances》 |2016年第107期|共11页
  • 作者

    Khatun Mahamuda; Choudhury Susobhan; Liu Bo; Lemmens Peter; Pal Samir Kumar; Mazumder Santasree;

  • 作者单位

    Univ Calcutta Ballygunge Sci Coll Dept Biochem Kolkata 700019 India;

    SN Bose Natl Ctr Basic Sci Dept Chem Biol &

    Macromol Sci Block JD Sect 3 Kolkata 700106 India;

    TU Braunschweig Inst Condensed Matter Phys Mendelssohnstr 3 D-38106 Braunschweig Germany;

    TU Braunschweig Inst Condensed Matter Phys Mendelssohnstr 3 D-38106 Braunschweig Germany;

    SN Bose Natl Ctr Basic Sci Dept Chem Biol &

    Macromol Sci Block JD Sect 3 Kolkata 700106 India;

    Univ Calcutta Ballygunge Sci Coll Dept Biochem Kolkata 700019 India;

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  • 中图分类 化学;
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