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首页> 外文期刊>RSC Advances >Ceratonia siliqua leaves exert a strong ROS-scavenging effect in human neutrophils, inhibit myeloperoxydase in vitro and protect against intestinal fluid and electrolytes secretion in rats
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Ceratonia siliqua leaves exert a strong ROS-scavenging effect in human neutrophils, inhibit myeloperoxydase in vitro and protect against intestinal fluid and electrolytes secretion in rats

机译：Ceratonia Siliqua叶子在人性嗜中性粒细胞中施加强烈的ROS扫荡作用，体外抑制肌释放酶并防止肠道液和电解质在大鼠中分泌

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摘要

Chronic inflammation and oxidative stress are induced by biological, chemical and physical factors which are, in turn, associated with an increased risk of several human diseases. Plants are a large source of new bioactive molecules with therapeutic potential. In this respect, the present study was designed to investigate the effects of a Ceratonia siliqua L. leaf aqueous extract (CSLAE) on human neutrophil reactive oxygen species (ROS) production, in vitro myeloperoxidase (MPO) activity and expression as well as the small intestinal fluid and electrolyte secretion. Neutrophils were isolated from whole human blood of healthy volunteers using the ficoll-dextran method and ROS generation and H2O2 were measured by luminol amplified chemiluminescence. Superoxide anion generation was also detected by chemiluminescence using the lucigenin method. MPO activity and quantity were measured by the tetramethylbenzidine oxidation method and Western blotting analysis. Concerning the in vivo part, fasted male rats received by gavage either the vehicle (NaCl, 0.9%), the extract at various doses (50, 100 and 200 mg kg(-1)) or clonidine (1 mg kg(-1)). An activated charcoal suspension was administered by oral gavage. Thirty minutes after receiving the charcoal meal, rats were euthanized and the small intestine was removed. The length of the small intestine and the distance traveled by the charcoal were recorded. Castor oil-induced hypersecretion in Wistar rats was treated with administration of CSLAE (50, 100 and 200 mg kg(-1)) and antidiarrheal drug, Atropine (0.1 mg kg(-1) i.p.). CSLAE inhibited luminol-amplified chemiluminescence of (PMA)-stimulated neutrophils in a concentration-dependent manner and is able to scavenge superoxide anions and hydrogen peroxide. The CSLAE also reduces significantly and dose-dependently MPO activity and expression. On the other hand, in vivo studies showed that the CSLAE decreased notably and dose-dependently the GIT activity, intestinal fluid and electrolyte concentration. The chemical analysis using a HPLC technique showed that the CSLAE is rich in phenolic compounds, especially kaempferol, tannic acid and catechin hydrate. The intended neutrophil inhibition is introduced as a part of a new strategy for pharmacological modulation of chronic inflammatory and oxidative stress processes. On the basis of these findings, it can be also assumed that CSLAE could be a potential source for novel discovery for antidiarrhoeal drug development.
机译：慢性炎症和氧化应激是通过生物，化学和物理因素诱导的，又与几种人类疾病的风险增加相关。植物是具有治疗潜力的新型生物活性分子的大来源。在这方面，本研究旨在探讨Ceratonia siliqua L.叶含水提取物（Cslae）对人性粒细胞反应性氧（ROS）生产，体外髓过氧化物酶（MPO）活性和表达以及小的影响肠液和电解质分泌。使用Ficoll-Dextran方法从健康志愿者的整个人类血液中分离中性粒细胞，并通过Luminol扩增的化学发光测量ROS生成和H 2 O 2。使用Lucigenin方法还通过化学发光检测超氧化物阴离子。通过四甲基苯甲酸氧化方法和蛋白质印迹分析测量MPO活性和量。关于体内部分，通过饲养载体（NaCl，0.9％），提取物的禁食雄性大鼠，以各种剂量（50,100和200mg kg（-1））或克里纳尼（1mg kg（-1） ）。通过口服饲料施用活性炭悬浮液。收到木炭膳食后三十分钟，大鼠安乐死，小肠被除去。记录小肠的长度和木炭行驶的距离。蓖麻油诱导的Wistar大鼠的过度分泌物用Cslae（50,100和200mg kg（-1））和抗原始药物，阿托品（0.1mg kg（-1）I.p.）治疗。 Cslae以浓度依赖性方式抑制（PMA）刺激的中性粒细胞的浓酚扩增化学发光，并且能够清除超氧化物阴离子和过氧化氢。 CSLAE还显着和剂量依赖性MPO活性和表达减少。另一方面，在体内研究表明，CSLAE显着下降，依赖性地减少了Git活性，肠液和电解质浓度。使用HPLC技术的化学分析表明，CSLAE富含酚类化合物，尤其是Kaempferol，单宁酸和儿茶素水合物。引入了预期的中性粒细胞抑制作为慢性炎症和氧化应激过程的药理调节的新策略的一部分。在这些发现的基础上，也可以假设CSLAE可以是对抗神经性药物发育的新发现的潜在来源。

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  • 来源
    《RSC Advances》 |2016年第70期|共11页
  • 作者

    Rtibi Kais; Jabri Mohammed-Amine; Selmi Slimen; Sebai Hichem; Amri Mohamed; El-Benna Jamel; Marzouki Lamjed;

  • 作者单位

    Fac Sci Tunis Dept Sci Biol Lab Neurophysiol Fonct &

    Pathol Campus Univ El Manar 2 Tunis 2092 Tunisia;

    Inst Super Biotechnol Beja Lab Nutr &

    Physiol Anim Ave Habib Bourguiba BP 382-9000 Beja Tunisia;

    Inst Super Biotechnol Beja Lab Nutr &

    Physiol Anim Ave Habib Bourguiba BP 382-9000 Beja Tunisia;

    Inst Super Biotechnol Beja Lab Nutr &

    Physiol Anim Ave Habib Bourguiba BP 382-9000 Beja Tunisia;

    Fac Sci Tunis Dept Sci Biol Lab Neurophysiol Fonct &

    Pathol Campus Univ El Manar 2 Tunis 2092 Tunisia;

    Univ Paris 07 INSERM U773 Ctr Rech Biomed F-75018 Paris France;

    Fac Sci Tunis Dept Sci Biol Lab Neurophysiol Fonct &

    Pathol Campus Univ El Manar 2 Tunis 2092 Tunisia;

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  • 正文语种 eng
  • 中图分类 化学;
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