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首页> 外文期刊>RSC Advances >Simple and signal-off electrochemiluminescence immunosensor for alpha fetoprotein based on gold nanoparticle-modified graphite-like carbon nitride nanosheet nanohybrids
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Simple and signal-off electrochemiluminescence immunosensor for alpha fetoprotein based on gold nanoparticle-modified graphite-like carbon nitride nanosheet nanohybrids

机译：基于金纳米颗粒改性石墨类氮化物纳米液体的α胎儿蛋白的简单且信号脱硫电化学传感器

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摘要

A simple, signal-off electrochemiluminescence (ECL) immunosensor for sensitive and selective detection of alpha fetoprotein (AFP) was developed based on gold nanoparticle (AuNPs) modified graphite-like carbon nitride nanosheet (g-C3N4 NSs) nanohybrids (Au-g-C3N4 NHs). Compared to the g-C3N4 NS modified glassy carbon electrode (GCE), the ECL intensity is more obvious at the Au-g-C3N4 NH modified GCE due to the fact that AuNPs can promote electron transfer and electrocatalytic reduction of S2O82- to produce large amounts of hole donor (SO4 center dot-). Results demonstrated that the ECL signal of the g-C3N4 NSs decreased due to the interaction between antibody and antigen on the modified electrode. Thus, an ECL immunosensor for the detection of AFP was realized by monitoring the ECL intensity change of g-C3N4 NSs. The factors influencing the performance of the immunosensor were investigated in detail. Under optimal conditions, the proposed biosensor achieved a wide line from 0.001 to 5.0 ng mL(-1) with a detection limit of 0.0005 ng mL(-1). Furthermore, the ECL immunosensor was successfully applied to the determination of AFP in serum samples, providing a promising effective strategy for AFP detection.
机译：用于敏感和选择性检测α胎儿蛋白（AFP）的简单，信号关闭电化学传感器（ECL）的免疫传感器是基于金纳米粒子（AUNP）改性石墨氮化物纳米晶片（G-C3N4 NSS）纳米嗜含量（AU-G- C3n4 NHS）。与G-C3N4 NS改性玻璃电极（GCE）相比，由于AUNPS可以促进电子转移和电催化还原S2O82-产生大的事实，ECL强度在AU-G​​-C3N4 NH改性GCE中更为明显孔供体（SO4中心点）的数量。结果表明，G-C3N4NSS的ECL信号由于抗体与修饰电极上的抗原之间的相互作用而降低。因此，通过监测G-C3N4 NSS的ECL强度变化来实现用于检测AFP的ECL免疫传感器。对影响免疫传感器性能的因素进行了详细研究。在最佳条件下，所提出的生物传感器达到0.001至5.0ng ml（-1）的宽线，检测限为0.0005 ng ml（-1）。此外，ECL免疫传感器已成功应用于血清样品中AFP的测定，为AFP检测提供了有希望的有效策略。

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  • 来源
    《RSC Advances》 |2016年第26期|共9页
  • 作者

    Zheng Xiangli; Hua Xiaoxia; Qiao Xueying; Xia Fangquan; Tian Dong; Zhou Changli;

  • 作者单位

    Univ Jinan Key Lab Chem Sensing &

    Anal Univ Shandong Jinan 250022 Peoples R China;

    Univ Jinan Key Lab Chem Sensing &

    Anal Univ Shandong Jinan 250022 Peoples R China;

    Univ Jinan Key Lab Chem Sensing &

    Anal Univ Shandong Jinan 250022 Peoples R China;

    Univ Jinan Key Lab Chem Sensing &

    Anal Univ Shandong Jinan 250022 Peoples R China;

    Univ Jinan Key Lab Chem Sensing &

    Anal Univ Shandong Jinan 250022 Peoples R China;

    Univ Jinan Key Lab Chem Sensing &

    Anal Univ Shandong Jinan 250022 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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