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Cross-linked silk sericin-gelatin 2D and 3D matrices for prospective tissue engineering applications

机译：交联丝丝氨酸 - 明胶2D和3D矩阵用于预期组织工程应用

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摘要

Biomaterials play an important role as templates to mimic native extracellular matrices in tissue engineering applications. Of late, attributes such as mitogenic activity, biocompatibility and biodegradability have conferred a special niche to silk sericin (SS) in the field of tissue engineering. This study deals with the fabrication of two dimensional (2D) and three dimensional (3D) matrices based on blending of gelatin with SS extracted from the cocoons of Bombyx mori (BM), Antheraea assamensis (AA) and Philosamia ricini (PR) silkworms. The fabricated matrices were cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Assessment of surface topography using atomic force microscopy (AFM) revealed enhanced surface roughness of SS post blending with gelatin. The cross-linking mediated secondary structural changes were confirmed by Fourier transform infrared spectroscopy (FTIR). Gelatin/PR sericin (GPRS) blended 3D matrices were found to be highly porous compared to gelatin/BM sericin (GBMS), gelatin/AA sericin (GAAS) and only gelatin matrices. In vitro protein release studies showed maximum amount of SS release from GAAS matrices. Blending of SS with gelatin lowered the mechanical strength of the matrices. Cells (mouse fibroblast and human keratinocytes) cultured on gelatin/silk sericin (GSS) matrices showed enhanced attachment and proliferation. Furthermore, flow cytometric cell cycle analysis attested that the matrices supported cell growth without any cell cycle arrest. The amount of tumor necrosis factor (TNF)-alpha released by murine macrophage (Raw 264.7) cells cultured on GSS matrices suggested immunocompatibility of the fabricated matrices. These findings corroborate that the GSS 2D and 3D matrices may serve as prospective biomaterials in the domain of tissue engineering.
机译：生物材料在组织工程应用中以模拟原生细胞外基质的模板发挥重要作用。最近，含有丝肠病活性，生物相容性和生物降解性的属性已经在组织工程领域赋予丝丝蛋白（SS）的特殊乳蛋白。该研究涉及基于从Bombyx Mori（BM），Antheraea issamensis（AA）和Philosamia Ricini（PR）蚕的茧中提取的明胶的二维（2D）和三维（3D）矩阵的制造。使用1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）/ N-羟基琥珀酰亚胺（NHS）交联所述基质。使用原子力显微镜（AFM）的表面形貌评估显示SS柱与明胶的增强表面粗糙度。通过傅里叶变换红外光谱（FTIR）确认交联介导的二次结构变化。与明胶/ BM胺（GBMS），明胶/ AA胺（GaAs）和仅明胶基质相比，发现明胶/ Pr Sericin（GPRS）混合3D基质是高度多孔的。体外蛋白质释放研究显示来自GaAs基质的最大SS释放量。用明胶的SS混合降低了基质的机械强度。在明胶/丝胺（GSS）基质上培养的细胞（小鼠成纤维细胞和人角蛋白细胞显示出增强的附着和增殖。此外，流式细胞术细胞循环分析证明基质支持细胞生长而不进行任何细胞周期停滞。由鼠巨噬细胞释放的肿瘤坏死因子（TNF） - 在GSS基质上培养的细胞释放的肿瘤坏死因子（RAW 264.7）细胞提出了制造基质的免疫相容性。这些发现证实了GSS 2D和3D矩阵可以用作组织工程领域的前瞻性生物材料。

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  • 来源
    《RSC Advances》 |2016年第107期|共12页
  • 作者

    Kumar Jadi Praveen; Bhardwaj Nandana; Mandal Biman B.;

  • 作者单位

    Indian Inst Technol Dept Biosci &

    Bioengn Biomat &

    Tissue Engn Lab Gauhati 781039 Assam India;

    IASST Div Life Sci Gauhati 781035 Assam India;

    Indian Inst Technol Dept Biosci &

    Bioengn Biomat &

    Tissue Engn Lab Gauhati 781039 Assam India;

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