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Systemically replicated organic and inorganic bony microenvironment for new bone formation generated by a 3D printing technology

机译：用于3D印刷技术产生的新骨形成的系统性复制的有机和无机骨髓微环境

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摘要

Here, bone demineralized and decellularized extracellular matrix (bdECM) was coated on the surface of the 3D printed polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and beta-tricalcium phosphate (TCP) mixture scaffolds (PCL/PLGA/TCP) that induce in vitro osteogenic activity and in vivo critical sized bone defect healing. Collagen coating on PCL/PLGA/TCP was used as a positive control. bdECM coating on PCL/PLGA/TCP successfully proceeded maintaining its pore interconnective structure. PCL/PLGA/TCP/bdECM showed the highest osteoblast adhesion with alignment morphology. These enhanced cell adhesion and alignment might contribute to in vitro osteogenic activity of osteoblasts combined with osteogenic biomolecules in bdECM. Furthermore, in vivo bone defect healing was enhanced compared to the other scaffolds. Three type of scaffold (PCL/PLGA/TCP, PCL/PLGA/TCP/Col, and PCL/PLGA/TCP/bdECM) with or without osteoblasts were implanted into the mouse calvarial defect. In vivo bone healing was measured by microcomputed topography and histological staining. PCL/PLGA/TCP with or without osteoblast implantation on the mouse calvarial defect only showed fibrous tissue formation. PCL/PLGA/TCP/Col with osteoblasts showed the half ratio of bone healing. However PCL/PLGA/TCP/bdECM without osteoblasts showed significantly enhanced bone defect healing. This result indicated that PCL/PLGA/TCP/bdECM is sufficient to induce the critical bone defect healing due to the bdECM containing biomolecules and enhanced host cell ingrowth into the defect.
机译：这里，骨脱耳化和脱细胞外细胞外基质（BDECM）涂覆在3D印刷聚己内酯（PCL）的表面上，聚（乳酸二乙醇酸）（PLGA）和β-三钙（TCP）混合物支架（PCL / PLGA / TCP）诱导体外成骨活性和体内临界大小骨缺损愈合。 PCL / PLGA / TCP上的胶原涂层用作阳性对照。 PCL / PLGA / TCP上的BDECM涂层成功进行了维持其孔隙互连结构。 PCL / PLGA / TCP / BDECM显示出最高的成骨细胞粘附与对准形态。这些增强的细胞粘附和对准可能有助于成骨细胞的体外成骨活性与BDECM中的骨质形成生物分子联合。此外，与其他支架相比，体内骨缺损愈合增强。用或不具有成骨细胞的三种类型的支架（PCL / PLGA / TCP，PCL / PLGA / TCP / COL和PCL / PLGA / TCP / BDCM）植​​入小鼠颅骨缺陷中。通过微锁定形貌和组织学染色测量体内骨愈合。 PCL / PLGA / TCP具有或没有成骨细胞植入小鼠颅骨缺陷仅显示纤维组织形成。具有成骨细胞的PCL / PLGA / TCP / COL显示出骨愈合的一半比例。然而，没有成骨细胞的PCL / PLGA / TCP / BDECM显示出显着增强的骨缺损愈合。该结果表明，由于含有Bdecm的生物分子和增强宿主细胞到缺陷，PCL / PLGA / TCP / BDECM足以诱导临界骨缺损愈合。

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  • 来源
    《RSC Advances》 |2016年第14期|共8页
  • 作者

    La Wan-Gun; Jang Jinah; Kim Byoung Soo; Lee Min Suk; Cho Dong-Woo; Yang Hee Seok;

  • 作者单位

    Dankook Univ Dept Nanobiomed Sci Cheonan 330714 South Korea;

    Pohang Univ Sci &

    Technol POSTECH Dept Mech Engn Pohang 790784 South Korea;

    Pohang Univ Sci &

    Technol POSTECH Dept Mech Engn Pohang 790784 South Korea;

    Dankook Univ Dept Nanobiomed Sci Cheonan 330714 South Korea;

    Pohang Univ Sci &

    Technol POSTECH Dept Mech Engn Pohang 790784 South Korea;

    Dankook Univ Dept Nanobiomed Sci Cheonan 330714 South Korea;

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