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首页> 外文期刊>RSC Advances >Hyaluronic acid/EDC/NHS-crosslinked green electrospun silk fibroin nanofibrous scaffolds for tissue engineering
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Hyaluronic acid/EDC/NHS-crosslinked green electrospun silk fibroin nanofibrous scaffolds for tissue engineering

机译：透明质酸/ EDC / NHS交联绿色电纺丝丝纤维蛋白纳米纤维组织工程支架

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摘要

Regenerated silk fibroin (SF) from Bombyx mori was used widely in biomedical fields due to its excellent properties. In recent years, green electrospun SF nanofibers have attracted much attention from researchers due to their good biosafety and environmentally friendly nature. However, the mechanical properties of SF nanofibers are unsatisfactory, which greatly restricts the application of SF in tissue engineering. In this study, the tensile performance of the green electrospun SF nanofibers was significantly improved through a simple and eco-friendly process of hyaluronic acid (HA)/1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)-crosslinking. Our data showed that the strain performance of the HA/EDC/NHS-crosslinked SF nanofibrous matrices was dramatically improved up to 120%, and was much better than that of the conventionally treated ones (ethanol soaking or fumigating). The appropriate HA/EDC/NHS-crosslinking time for SF nanofibrous matrices was 24 hours. The strain performance of the as-crosslinked SF nanofibers increased with an increase in HA concentration, with the optimal HA concentration being 0.3% (w/v). Fourier transform infrared spectroscopy analysis suggested that the HA/EDC/NHS-crosslinking process involves covalent reactions. The hydrophilicity of the SF nanofibrous matrices also increased with the addition of HA, which can be useful when trying to resist non-specific protein adsorption. In addition, the as-crosslinked SF nanofibrous matrices exhibited good cytocompatibility as shown by the cell viability data. Our work demonstrated that HA/EDC/NHS-crosslinking is a good choice for improving the tensile properties of the green electrospun SF nanofibers serving as skin tissue engineering scaffolds.
机译：由于其优异的性能，来自Bombyx Mori的再生丝素蛋白（SF）在生物医学领域中广泛使用。近年来，由于其良好的生物安全和环保性质，绿色电纺社SF纳米纤维吸引了研究人员的重视。然而，SF纳米纤维的机械性能不令人满意，这极大地限制了SF在组织工程中的应用。在这项研究中，通过透明质酸（HA）/ 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）/ N-羟基琥珀酰亚胺的简单和生态友好的工艺显着改善了绿色电纺SF纳米纤维的拉伸性能（NHS） - 克隆思考。我们的数据表明，HA / EDC / NHS交联的SF纳米纤维基质的应变性能显着提高至120％，并且比常规处理的（乙醇浸泡或熏蒸）要好得多。 SF纳米纤维基质的合适的HA / EDC / NHS交联时间为24小时。随着HA浓度的增加，AS交联的SF纳米纤维的应变性能增加，最佳HA浓度为0.3％（w / v）。傅里叶变换红外光谱分析表明，HA / EDC / NHS交联过程涉及共价反应。随着HA的添加，SF纳米纤维基质的亲水性也增加，这在试图抵抗非特异性蛋白质吸附时可以是有用的。另外，配交联的SF纳米纤维族基质表现出良好的细胞织地性，如细胞活力数据所示。我们的工作表明，HA / EDC / NHS交联是改善用作皮肤组织工程支架的绿色电纺SF纳米纤维的拉伸性能的良好选择。

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  • 来源
    《RSC Advances》 |2016年第102期|共9页
  • 作者

    Yang Xingxing; Wang Xiaoyun; Yu Fan; Ma Linlin; Pan Xiaohan; Luo Gejie; Lin Si; Mo Xiumei; He Chuanglong; Wang Hongsheng;

  • 作者单位

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 1 Dept Obstet &

    Gynecol Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

    Donghua Univ Coll Chem Chem Engn &

    Biotechnol Biomat &

    Tissue Engn Lab Shanghai 201620 Peoples R China;

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