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Cellulose as an efficient matrix for lipase and transaminase immobilization

机译：纤维素作为脂肪酶和转氨酶固定化的有效基质

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摘要

Immobilization of enzymes is important to improve their stability and to facilitate their recyclability, aiming to make biocatalytic processes more efficient. One of the important aspects is the utilization of cheap, abundant, and environmentally friendly carriers for enzyme immobilization. Here we report the use of functionalized cellulose for lipase and transaminase immobilization. High immobilization efficiencies (up to 90%) could be achieved for the transaminase from Vibrio fluvialis. For immobilized lipase CAL-B as well as the transaminase, good conversions and recyclability could be demonstrated in kinetic resolutions to afford chiral alcohols or amines. Moreover, such application of the immobilized transaminase enabled very high conversions in a continuous-flow process in the asymmetric synthesis of (S)-phenylethylamine (80% conversion, >99% ee).
机译：酶的固定化对于改善它们的稳定性是重要的，并促进其可回收性，旨在使生物催化过程更有效。 其中一个重要方面是利用廉价，丰富，环保的携带者进行酶固定化。 在这里，我们报告使用官能化纤维素用于脂肪酶和转氨酶固定化。 从vibrio flyvialis的转氨酶可以实现高固定效率（高达90％）。 对于固定化脂肪酶Cal-B以及转氨酶，可以以动力学分辨率证明良好的转化和可回收性，以提供手性醇或胺。 此外，这种固定的转氨酶的应用在不对称合成（S） - 苯乙胺（80％转化率，> 99％EE）中的连续流程中的连续流程中的非常高的转化。

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  • 来源
    《RSC Advances》 |2016年第8期|共7页
  • 作者

    de Souza Stefania P.; Junior Ivaldo I.; Silva Guilherme M. A.; Miranda Leandro S. M.; Santiago Marcelo F.; Lam Frank Leung-Yuk; Dawood Ayad; Bornscheuer Uwe T.; de Souza Rodrigo O. M. A.;

  • 作者单位

    Univ Fed Rio de Janeiro Inst Chem Biocatalysis &

    Organ Synth Grp Rio De Janeiro Brazil;

    Univ Rio de Janeiro Sch Chem Rio De Janeiro Brazil;

    Univ Fed Rio de Janeiro Inst Chem Biocatalysis &

    Organ Synth Grp Rio De Janeiro Brazil;

    Univ Fed Rio de Janeiro Inst Chem Biocatalysis &

    Organ Synth Grp Rio De Janeiro Brazil;

    Univ Fed Rio de Janeiro IBCCF Rio De Janeiro Brazil;

    Hong Kong Univ Sci &

    Technol Dept Chem &

    Biomol Engn Hong Kong Hong Kong Peoples R China;

    Ernst Moritz Arndt Univ Greifswald Inst Biochem Dept Biotechnol &

    Enzyme Catalysis Greifswald Germany;

    Ernst Moritz Arndt Univ Greifswald Inst Biochem Dept Biotechnol &

    Enzyme Catalysis Greifswald Germany;

    Univ Fed Rio de Janeiro Inst Chem Biocatalysis &

    Organ Synth Grp Rio De Janeiro Brazil;

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