A photoactivatable Src homology 2 (SH2) domain

Song X.; Shang X.; Ju T.; Cerny R.; Niu W.; Guo J.

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A photoactivatable Src homology 2 (SH2) domain

机译：PhotoCtivatable SRC同源2（SH2）域

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摘要

Src homology 2 (SH2) domains bind specifically to phosphotyrosine-containing motifs. As a regulatory module of intracellular signaling cascades, the SH2 domain plays important roles in the signal transduction of receptor tyrosine kinase pathways. In this work, we reported the construction of a photoactivatable SH2 domain through the combination of protein engineering and genetic incorporation of photo-caged unnatural amino acids. Significantly enhanced recognition of a phosphotyrosine-containing peptide substrate by the engineered SH2 domain mutant was observed after light-induced removal of the photo-caging group. Optical activation allows the control of protein and/or cellular function with temporal and spatial resolution. This photoactivatable SH2 domain could potentially be applied to the study of tyrosine phsophorylation-associated biological processes.

机译：SRC同源性2（SH2）结构域特异性结合含磷酸丝氨酸的基序。作为细胞内信号传导级联的调节模块，SH2结构域在受体酪氨酸激酶途径的信号转导中起重要作用。在这项工作中，我们通过蛋白质工程和遗传掺入的光笼式非自然氨基酸的组合报道了一种光活化的SH2结构域。在光诱导的去除光持续的光盘组之后，观察到通过工程化SH2结构域突变体显着增强含磷酸酪氨酸肽底物的识别。光学激活允许使用时间和空间分辨率控制蛋白质和/或蜂窝功能。该光活化的SH2结构域可能适用于酪氨酸phsophoration相关的生物过程的研究。

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《RSC Advances》 |2016年第56期|共5页
作者
Song X.; Shang X.; Ju T.; Cerny R.; Niu W.; Guo J.;
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Univ Nebraska Dept Chem Lincoln NE 68588 USA;

Univ Nebraska Dept Chem Lincoln NE 68588 USA;

Univ Nebraska Dept Chem Lincoln NE 68588 USA;

Univ Nebraska Dept Chem Lincoln NE 68588 USA;

Univ Nebraska Dept Chem &

Biomol Engn Lincoln NE 68588 USA;

Univ Nebraska Dept Chem Lincoln NE 68588 USA;

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正文语种 eng
中图分类化学;
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专利

1. A photoactivatable Src homology 2 (SH2) domain [J] . Song X., Shang X., Ju T., RSC Advances . 2016,第56期

机译：PhotoCtivatable SRC同源2（SH2）域
2. Src homology region 2 (SH2) domain-containing phosphatase-1 dephosphorylates B cell linker protein/SH2 domain leukocyte protein of 65 kDa and selectively regulates c-Jun NH2-terminal kinase activation in B cells. [J] . Mizuno K, Tagawa Y, Mitomo K, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2000,第3期

机译：含Src同源区域2（SH2）域的磷酸酶-1使65 kDa的B细胞接头蛋白/ SH2域白细胞蛋白脱磷酸化，并选择性调节B细胞中c-Jun NH2末端激酶的活化。
3. Antagonists of the Src homology 2 (SH2) domains of Grb2, Src, Lck and ZAP-70. [J] . Garcia-Echeverria C, Garcia-Echeverria C Current medicinal chemistry . 2001,第13期

机译：Grb2，Src，Lck和ZAP-70的Src同源2（SH2）域的拮抗剂。
4. Design of Phosphotyrosine Mimetics Targeting the Src Homology 2 Domain-Containing Phosphatase 1 (SHP-1) Non-Receptor Binding Site [C] . Kulis C., Bourne G.T., Kellie S. International Peptide Symposium . 2009

机译：靶向含SRC同源性2结构域的磷酸酶1（SHP-1）非受体结合位点的磷酸吡塞胺模拟物的设计
5. Disulfide Bridging the Gap between Src and Cortactin: A New Paradigm in SH2 Domain-mediated Signaling. [D] . Evans, Jason VanBuren. 2013

机译：二硫键弥合Src和Cortactin之间的差距：SH2域介导的信号传导的新范式。
6. Adapter protein with a pleckstrin homology (PH) and an Src homology 2 (SH2) domain (APS) and SH2-B enhance insulin-receptor autophosphorylation extracellular-signal-regulated kinase and phosphoinositide 3-kinase-dependent signalling. [O] . Zamal Ahmed, Tahir S Pillay 2003

机译：具有pleckstrin同源性（PH）和Src同源性2（SH2）域（APS）和SH2-B的衔接蛋白可增强胰岛素受体的自身磷酸化细胞外信号调节的激酶和磷酸肌醇3激酶依赖性信号传导。
7. A photoactivatable Src homology 2 (SH2) domain [O] . Song, X., Shang, X., Ju, T., 2016

机译：可光活化的src同源物2（sH2）结构域

A photoactivatable Src homology 2 (SH2) domain

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