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首页> 外文期刊>RSC Advances >A sensitivity-enhanced heterologous immunochromatographic assay based on a monoclonal antibody for the rapid detection of histamine in saury samples
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A sensitivity-enhanced heterologous immunochromatographic assay based on a monoclonal antibody for the rapid detection of histamine in saury samples

机译：基于单克隆抗体的敏感性增强的异源免疫色谱测定，用于在Saury样品中快速检测组胺的快速检测

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摘要

Histamine (HA) is an essential test item for fishery samples. However, the fast and effective determination of HA is difficult due to its simple structure and small molecular size. In this study, a sensitive and specific monoclonal antibody against p-nitrobenzoylated histamine (NPHA), which can be easily obtained from the reaction of HA and p-nitrobenzoic acid N-hydroxysuccinimide ester (PNBA-OSu) under mild conditions, was generated for the first time. Based on this mAb, an immunochromato-graphic assay strip (ICA strip) using a colloidal gold nano particles-antibody (GNPs) probe for rapid detection of HA in saury samples was established. After screening the coating antigens and optimization of analytical parameters, a heterologous coating based ICA strip exhibited the most excellent detection ability with a visual detection limit (VDL) of 6.0 mg kg(-1) in qualitative experiments and a detection limit (by a strip reader) of 1.0 mg kg(-1) in semi-quantitative experiments for saury samples, with no cross-reactivity with HA analogs. Good correlation between the ICA strip with liquid chromatography-tandem mass spectrometry was achieved for spiked and naturally contaminated saury samples. Overall, this method is suitable for screening of HA residue for large scale fish samples in a quick, simple and low-cost manner.
机译：组胺（HA）是渔业样本的必要测试项目。然而，由于其结构简单和小分子尺寸，对HA的快速有效测定是困难的。在该研究中，产生抗p-硝基苯甲酸酯组胺（NPHA）的敏感和特异的单克隆抗体，其可以在低别条件下从HA和P-硝基苯甲酸N-羟琥珀酰亚胺酯（PNBA-OSU）的反应中容易地获得。第一次。基于该MAB，使用胶体金纳米颗粒 - 抗体（GNPS）探针在Saury样品中快速检测HA的快速检测的免疫粒子 - 图形测定条（ICA条）。在筛选涂层抗原和分析参数的优化后，基于异源涂层的ICA条带，在定性实验中具有6.0mg kg（-1）的视觉检测限（Vdl）和检测极限（条带读者）在半定量实验中为Saury样品的半定量实验，没有与HA类似物的交叉反应性。为掺入和天然受污染的Saury样品实现了ICA条带液相色谱 - 串联质谱之间的良好相关性。总体而言，这种方法适用于以快速，简单，低成本的方式筛选大规模鱼类样品的HA残留物。

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  • 来源
    《RSC Advances》 |2015年第96期|共8页
  • 作者

    Luo Lin; Yang Jin-Yi; Xiao Zhi-Li; Zeng Dao-Ping; Li Yong-Jun; Shen Yu-Dong; Sun Yuan-Ming; Lei Hong-Tao; Wang Hong; Xu Zhen-Lin;

  • 作者单位

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    Guangzhou Wanlian Biotechnol Co Ltd Guangzhou 510670 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Guangdong Prov Key Lab Food Qual &

    Safety Guangzhou 510642 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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