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Efficient degradation of sulfamethoxazole and the response of microbial communities in microbial fuel cells

机译:高效降解磺胺甲恶唑和微生物燃料电池中微生物群落的响应

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摘要

Sulfamethoxazole (SMX) is an extensively consumed sulfonamide antimicrobial agent and is frequently detected in surface water. This work studied the degradation process of SMX in anodic chamber of microbial fuel cell (MFC) reactors. We found that the biodegradation of SMX could be achieved after acclimation and even high concentrations of SMX (e.g. 200 ppm) could be rapidly degraded. Excitation and emission matrix fluorescence spectroscopy analysis revealed that the chemical structure of SMX was altered during the process. Q-Exactive hybrid quadrupole-Orbitrap mass spectrometry was used to identify the degradation byproducts of SMX. The activity of electrode biofilm was examined afterwards and it was found that the microbe was in an active state. High-throughput sequencing analysis suggested that the microbial community structure was greatly changed during the process; some reported SMX scavengers, such as Achromobacter and Pseudomonas, were abundant in the reactors. Some metazoans were also recognized in the biofilm samples, which indicates that the operation of the MFC reactors was in a steady state. This study discusses the degradation mechanism of SMX and explores the microbial community response during the process, which provides useful information for the application of MFC in antibiotic elimination.
机译:磺胺甲恶唑(SMX)是广泛消耗的磺酰胺抗微生物剂,并且经常在地表水中检测。这项工作研究了微生物燃料电池(MFC)反应器阳极腔室中SMX的降解过程。我们发现,在适应且甚至高浓度的SMX(例如200ppm)之后,可以实现SMX的生物降解可以迅速降解。激发和发射基质荧光光谱分析显示,在该过程中改变了SMX的化学结构。 Q-辐射杂交四酮 - 赤渣质谱法用于鉴定SMX的降解副产物。然后检查电极生物膜的活性,发现微生物处于活性状态。高通量测序分析表明,在该过程中大大改变了微生物群落结构;一些报告的SMX清除剂如achroMobacter和假单胞菌,在反应堆中丰富。在生物膜样品中也识别出一些美唑烷,这表明MFC反应器的操作处于稳定状态。本研究讨论了SMX的降解机制,并探讨了过程中的微生物群落反应,为抗生素消除中的应用提供了有用的信息。

著录项

  • 来源
    《RSC Advances》 |2015年第69期|共8页
  • 作者单位

    Chinese Acad Sci Inst Urban Environm Key Lab Urban Pollutant Convers Xiamen 361021 Peoples R China;

    Xiamen Univ Technol Xiamen 361021 Peoples R China;

    Chinese Acad Sci Inst Urban Environm Key Lab Urban Pollutant Convers Xiamen 361021 Peoples R China;

    Chinese Acad Sci Inst Urban Environm Key Lab Urban Pollutant Convers Xiamen 361021 Peoples R China;

    Chinese Acad Sci Inst Urban Environm Key Lab Urban Pollutant Convers Xiamen 361021 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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