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Paper-based analytical device with colorimetric assay application to the determination of phenolic acids and recognition of Fe3+

机译:基于纸的分析装置,具有比色测定施用酚酸和Fe3 +识别的施用

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摘要

Considering the fact that phenolic acids (PAs) have a versatile activity in vivo and are found to be the most abundant antioxidants in our diet, their detection and quantification is very important for a healthy diet. Herein, we first propose a paper-based analytical device (PAD) for colorimetric detection of PAs and recognition of Fe3+. The PAD is fabricated based on a simple microarray. Specific probes are placed in the test zones on the PAD, followed by the introduction of assay targets. Color response and intensity measurements are directly conducted in the test zones after the completion of the probe-to-target reactions, avoiding the time-consuming integration of external devices and the involvement of chromophoric reagents. The PAD features easy fabrication and operation, low cost, and satisfied sensitivity, which is beneficial for the simple and fast detection of biomolecules.
机译:考虑到酚醛酸(PAS)在体内具有多功能活性,并且被发现是我们饮食中最丰富的抗氧化剂,它们的检测和定量对于健康饮食非常重要。 这里,我们首先提出了一种基于纸的分析装置(PAD),用于PAS的比色检测和Fe3 +的识别。 垫是基于简单的微阵列制造的。 将特定探针放置在垫上的测试区中,然后引入测定靶标。 在完成探针到目标反应后,在测试区中直接在测试区中直接进行颜色响应和强度测量,避免外部装置的耗时的整合和发色试剂的累积。 垫具有易于制造和操作,低成本和满意的灵敏度,这对于生物分子的简单和快速检测有益。

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  • 来源
    《RSC Advances》 |2015年第4期|共5页
  • 作者单位

    Chinese Acad Agr Sci Inst Oil Crops Res Hubei Key Lab Lipid Chem &

    Nutr Dept Prod Proc &

    Nutriol Minist Agr Key Lab Oil C Wuhan 430062 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Key Lab Analyt Chem Biol &

    Med Minist Educ Wuhan 430072 Peoples R China;

    Hubei Acad Agr Sci Inst Agroprod Proc &

    Nucl Agr Technol Wuhan 430064 Hubei Province Peoples R China;

    Chinese Acad Agr Sci Inst Oil Crops Res Hubei Key Lab Lipid Chem &

    Nutr Dept Prod Proc &

    Nutriol Minist Agr Key Lab Oil C Wuhan 430062 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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