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BODIPY-based fluorescent probe for the simultaneous detection of glutathione and cysteine/homocysteine at different excitation wavelengths

机译：基于BODIPY的荧光探针，用于在不同激发波长下同时检测谷胱甘肽和半胱氨酸/同型内膜

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摘要

We reported a BODIPY-based fluorescent probe for the simultaneous detection of GSH and Cys/Hcy. The nitrothiophenol moiety of the probe serves not only as a leaving group for the thiol-substitution reaction, but also a fluorescence quencher to provide a low emission background. The electron-withdrawing imidazolium group drastically increases reactivity of the Cys/Hcy-induced substitution-rearrangement reaction. The imidazolium group can further be replaced by GSH and resulted in a bithioether-product (lambda(abs) = 568 nm, lambda(em) = 588 nm), which showed distinct photophysical properties from the amino-product (lambda(abs) = 443 nm, lambda(em) = 530 nm) in the case of Cys/Hcy. It is noted that they exhibited great differences in absorption spectra of more than 120 nm. Thus, the simultaneous detection of GSH and Cys/Hcy can be achieved at different excitation wavelengths. The probe can quantitatively determinate the amount of Cys, Hcy and GSH in certain concentration ranges. We also found that the probe could detect GSH and Cys in living cells from different emission channels.
机译：我们报道了一种基于Bodipy的荧光探针，用于同时检测GSH和Cys / Hcy。探针的硝基酚部分不仅可以作为硫醇取代反应的离去基团，而且还用于提供低排放背景的荧光猝灭剂。吸电子咪唑鎓组大大增加了Cys / Hcy诱导的替代重排反应的反应性。咪唑鎓基团可以进一步由GSH代替，并产生氨基醚 - 产物（λ（ABS）= 568nm，λ（em）= 588nm），其显示出来自氨基产物的不同的光性特性（Lambda（ABS）=在Cys / Hcy的情况下，443 nm，lambda（em）= 530nm）。注意到，它们表现出大于120nm以上的吸收光谱的差异。因此，可以在不同的激发波长下同时检测GSH和CYS / HCY。探针可以定量地确定在某些浓度范围内的Cys，Hcy和Gsh的量。我们还发现探针可以从不同排放通道中检测生物细胞中的GSH和CYS。

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  • 来源
    《RSC Advances》 |2015年第6期|共6页
  • 作者

    Niu Li-Ya; Yang Qing-Qing; Zheng Hai-Rong; Chen Yu-Zhe; Wu Li-Zhu; Tung Chen-Ho; Yang Qing-Zheng;

  • 作者单位

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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