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Evaluation of imprint DESI-MS substrates for the analysis of fungal metabolites

机译：评价压印脱硫底物用于分析真菌代谢物

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摘要

Mass spectrometry (MS) has become an important tool in microbiology for the identification of microorganisms and for monitoring the production of microbial secondary metabolites. Therefore, several ambient mass spectrometry techniques have been applied in the last few years. Although desorption electrospray ionization mass spectrometry (DESI-MS) is the most popular ambient MS technique, its application in the direct analysis of fungal compounds is still not well established. The irregular or uneven nature of the colony, the absorbent properties of the growth medium and the need for a firm surface for effective ionization hinder the direct screening of metabolites in fungal cultures by DESI-MS analysis. To overcome these limitations, in this study, we tested different surfaces, such as tape, porous polytetrafluoroethylene (PTFE), thin layer chromatography (TLC) surfaces, filter paper, flat silicon wafers and porous silicon (pSi), to obtain the best imprint-DESI-MS outcome with fungal cultures of Trichoderma harzianum for metabolite screening. We also evaluated the efficiency of the surfaces for the DESI-MS of small polar fungal metabolites in regard to signal intensity, signal stability and imaging suitability. The silicon surfaces provided the highest signal intensity for the fungal metabolites. PTFE and filter paper demonstrated relatively high signal stabilities that could be useful for prolonged DESI-MS/MS experiments, whereas tape was found to be the best option for DESI-MS imaging. The imprint on a tape surface was the only one capable of maintaining the structural features of the concentric conidial rings of T. harzianum.
机译：质谱（MS）已成为微生物学中的重要工具，用于鉴定微生物和监测微生物二次代谢物的产生。因此，在过去几年中已经应用了几种环境质谱技术。尽管解吸电喷雾电离质谱（Desi-MS）是最受欢迎的环境MS技术，但其在真菌化合物的直接分析中的应用仍然没有很好地建立。菌落的不规则或不均匀性，生长培养基的吸收性和需要用于有效电离的坚固表面阻碍了通过Desi-MS分析在真菌培养物中的代谢物直接筛选。为了克服这些限制，在本研究中，我们测试了不同的表面，如胶带，多孔聚四氟乙烯（PTFE），薄层色谱（TLC）表面，滤纸，扁平硅晶片和多孔硅（PSI），以获得最佳印记-DESI-MS与Trichoderma Harzianum的真菌培养的结果进行代谢物筛选。我们还评估了对信号强度，信号稳定性和成像适宜性的小极性真菌代谢物的DESI-MS的表面的效率。硅表面为真菌代谢物提供了最高的信号强度。 PTFE和滤纸展示了对延长Desi-MS / MS实验有用的相对高的信号稳定性，而发现胶带是Desi-MS成像的最佳选择。胶带表面上的印记是唯一能够维持T. Harzianum的同心分析环的结构特征的印记。

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