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Reinforced chitosan beads by chitin nanofibers for the immobilization of beta-glucosidase

机译：用甲壳素纳米纤维增强壳聚糖珠，用于固定β-葡糖苷酶

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摘要

A chitosan (CS) solution was filled with partially deacetylated alpha-chitin nanofibers (DEChN) and the composite (DEChN/CS) beads prepared thereof were used for the immobilization of beta-glucosidase. The filling of chitin nanofibers resulted in an increase of the shear modulus (G', G ''), which indicated an improvement to the mechanical properties of the DEChN/CS composite beads. Meanwhile, the specific surface area was improved from 46.47 m(2) g(-1) to 229.71 m(2) g(-1) when the chitosan beads were filled with the chitin nanofibers, and the prepared DEChN/CS composite beads had a broad pore size ranging from 20 nm to 60 nm. Moreover, a more porous and fibrous structure in the SEM image of the DEChN/CS beads was observed compared to that of the sole CS beads. The chitin nanofibers provided a fibrous network in the chitosan matrix that enhanced both the mechanical and porous properties of the DEChN/CS composite beads, which is normally considered contradictory. All of these enhanced features improved the efficiency of enzyme immobilization from 40% in the chitosan (CS) beads to 67% in the DEChN/CS composite beads.
机译：将壳聚糖（Cs）溶液填充有部分脱乙酰化的α-丁蛋白纳米纤维（DECHN），并将其制备的复合物（DECHN / CS）珠粒用于固定β-葡糖苷酶。几丁质纳米纤维的填充导致剪切模量（G'，G''）增加，这表明对DeChn / CS复合珠的机械性能的改善。同时，当壳聚糖珠填充用丁蛋白纳米纤维填充时，从46.47m（2）g（-1）至229.71m（2）g（-1）提高了比表面积，并具有制备的DECHN / CS复合珠宽孔径范围为20nm至60nm。此外，与唯一的Cs珠粒相比，观察到DeChn / Cs珠粒的SEM图像中的更多孔和纤维结构。几丁质纳米纤维提供壳聚糖基质中的纤维网络，其增强了DECHN / CS复合珠的机械和多孔性质，通常认为是矛盾的。所有这些增强特征在DECHN / CS复合珠粒中，将酶固定化从壳聚糖（CS）珠粒中的40％提高到67％的酶。

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  • 来源
    《RSC Advances》 |2015年第113期|共6页
  • 作者

    Liu Liang; Lv Hechan; Jiang Jie; Zheng Ke; Ye Wenbo; Wang Zhiguo; Fan Yimin;

  • 作者单位

    Nanjing Forestry Univ Coll Chem Engn Jiangsu Key Lab Biomass Based Green Fuel &

    Chem Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Chem Engn Jiangsu Key Lab Biomass Based Green Fuel &

    Chem Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Chem Engn Jiangsu Key Lab Biomass Based Green Fuel &

    Chem Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Chem Engn Jiangsu Key Lab Biomass Based Green Fuel &

    Chem Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Chem Engn Jiangsu Key Lab Biomass Based Green Fuel &

    Chem Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Light Ind Sci &

    Engn Jiangsu Prov Key Lab Pulp &

    Paper Sci &

    Technol Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Chem Engn Jiangsu Key Lab Biomass Based Green Fuel &

    Chem Nanjing 210037 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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