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Coumarin-modified gold nanoprobes for the sensitive detection of caspase-3le

机译：Cocarin-改性的金纳罗基体，用于Caspase-3Le的敏感性检测

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摘要

Caspase-3 has been identified as a key mediator and a well-established cellular marker of apoptosis. To increase the sensitivity, coumarin-functionalized gold nanoparticles (AuNPs) connected to polypeptide chains containing specific sequences (DEVD) were designed and synthesized for the sensing of caspase-3, because there was a large overlap between the emission of coumarin-343 and the absorption of the AuNPs. The fluorescence of coumarin 343 was quenched due to the energy transfer process by the gold nanoparticles. The fluorescence could be restored after the particular polypeptide sequence (DEVD) was cut off by caspase-3. Based on this mechanism, the caspase enzyme activity in vitro could be detected by a fluorescence assay with a high sensitivity. The effect of the different lengths of polypeptide chains on the luminescence quenching efficiency and sensing ability was also studied, which is of great importance in designing FRET-based sensing platforms. This kind of sensitive luminescent
机译：Caspase-3已被鉴定为关键介体和良好的细胞凋亡细胞标志物。为了增加含有特定序列（DeVD）的Coumarin官能化金纳米颗粒（AUNP）被设计并合成Caspase-3的感测，因为香豆素-343的发射之间存在大重叠。吸收AUNPS。由于金纳米颗粒的能量转移过程，淬灭香豆素343的荧光。在通过Caspase-3切断特定的多肽序列（DeVD）之后，可以恢复荧光。基于该机制，可以通过具有高灵敏度的荧光测定来检测体外的胱天蛋白酶活性。研究了不同长度的多肽链对发光淬火效率和感测能力的影响，这在设计基于FRET的传感平台方面具有重要意义。这种敏感的发光

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  • 来源
    《RSC Advances》 |2015年第54期|共7页
  • 作者

    Wang Qiong; Hu Wenqin; Feng Qingyue; Cao Xiu-Hui; Chai Weiwei; Yi Changqing; Li Mei-Jin;

  • 作者单位

    Fuzhou Univ Dept Chem Fujian Prov Key Lab Anal &

    Detect Technol Food Sa Key Lab Anal &

    Detect Technol Food Safety Minist Fuzhou 350116 Peoples R China;

    Fuzhou Univ Dept Chem Fujian Prov Key Lab Anal &

    Detect Technol Food Sa Key Lab Anal &

    Detect Technol Food Safety Minist Fuzhou 350116 Peoples R China;

    Fuzhou Univ Dept Chem Fujian Prov Key Lab Anal &

    Detect Technol Food Sa Key Lab Anal &

    Detect Technol Food Safety Minist Fuzhou 350116 Peoples R China;

    Fuzhou Univ Dept Chem Fujian Prov Key Lab Anal &

    Detect Technol Food Sa Key Lab Anal &

    Detect Technol Food Safety Minist Fuzhou 350116 Peoples R China;

    Fuzhou Univ Dept Chem Fujian Prov Key Lab Anal &

    Detect Technol Food Sa Key Lab Anal &

    Detect Technol Food Safety Minist Fuzhou 350116 Peoples R China;

    Sun Yat Sen Univ Sch Engn Key Lab Sensing Technol &

    Biomed Instruments Guan Guangzhou 510006 Guangdong Peoples R China;

    Fuzhou Univ Dept Chem Fujian Prov Key Lab Anal &

    Detect Technol Food Sa Key Lab Anal &

    Detect Technol Food Safety Minist Fuzhou 350116 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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