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Current state of the art of mass spectrometry-based metabolomics studies - a review focusing on wide coverage, high throughput and easy identification

机译：基于质谱的代谢组研究的现状 - 一种重点覆盖，高吞吐量和易于识别的综述

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摘要

Metabolomics aims at the comprehensive assessment of a wide range of endogenous metabolites and attempts to identify and quantify the attractive metabolites in a given biological sample. These metabolites have diverse physicochemical properties and are presented at different concentration ranges, which makes global analysis a difficult challenge. As a commonly used analytical platform, mass spectrometry (MS) coupled with various separation techniques, such as gas chromatography (GC) and liquid chromatography (LC), has recently undergone rapid development, providing promising solutions to these problems. The ambient ionization techniques, including desorption electrospray ionization (DESI), direct analysis in real time (DART) and extractive electrospray ionization (EESI), enable rapid detection of metabolites, making it ideal for high-throughput analysis in large-scale metabolomics studies. The current applications of these approaches are described with selected illustrative examples in the present review. Furthermore, regardless of "targeted" or "non-targeted" metabolomics study, the identification of the attractive biomarkers is required to further interpret the related metabolic pathways. Therefore, in the present review, recent novel MS-based techniques that allow more robust and easier metabolite identification are summarized, and their strengths and [imitations are also discussed.
机译：代谢组科旨在综合评估各种内源性代谢物，并试图鉴定和量化给定的生物样品中具有吸引力的代谢物。这些代谢物具有不同的物理化学性质，并以不同的浓度范围呈现，这使全球分析成为一个艰难的挑战。作为常用的分析平台，与各种分离技术相结合的质谱（MS），例如气相色谱（GC）和液相色谱（LC），最近经历了快速的发展，为这些问题提供了有希望的解决方案。环境电离技术，包括解吸电喷雾电离（DESI），实时直接分析（DART）和萃取电喷雾电离（EESI），使得代谢物的快速检测，使其理想的大规模代谢组研究中的高通量分析。这些方法的当前应用描述于本综述中的所选说明性实施例。此外，无论“靶向”或“非靶向”的代谢组科研究，需要鉴定有吸引力的生物标志物，以进一步解释相关的代谢途径。因此，在本综述中，总结了允许更强大和更容易代谢物识别的基于基于MS的基于新的基于MS的技术，并且还讨论了它们的优点和[模拟。

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  • 来源
    《RSC Advances》 |2015年第96期|共10页
  • 作者

    Wang Yang; Liu Shuying; Hu Yuanjia; Li Peng; Wan Jian-Bo;

  • 作者单位

    Univ Macau State Key Lab Qual Res Chinese Med Inst Chinese Med Sci Taipa Macao Peoples R China;

    Changchun Univ Chinese Med Jilin Ginseng Acad Changchun Jilin Peoples R China;

    Univ Macau State Key Lab Qual Res Chinese Med Inst Chinese Med Sci Taipa Macao Peoples R China;

    Univ Macau State Key Lab Qual Res Chinese Med Inst Chinese Med Sci Taipa Macao Peoples R China;

    Univ Macau State Key Lab Qual Res Chinese Med Inst Chinese Med Sci Taipa Macao Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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