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首页> 外文期刊>RSC Advances >Influence of silver grain size, roughness, and profile on the extraordinary fluorescence enhancement capabilities of grating coupled surface plasmon resonance
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Influence of silver grain size, roughness, and profile on the extraordinary fluorescence enhancement capabilities of grating coupled surface plasmon resonance

机译：银粒度，粗糙度和谱对光栅耦合表面等离子体共振的非凡荧光增强能力的影响

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摘要

Since the development of fluoroimmunoassays, researchers have sought a method of substantially enhancing fluorescence intensity to extend the limits of detection to new levels of sensitivity. Surface plasmon resonance (SPR) and metal enhanced fluorescence has long been a topic of research and has led to the development of prism- and grating-based SPR systems. However, with the wide coupling range and ease of exciting SPR on plasmonic gratings with a simple microscope objective, they have tremendous potential for revolutionizing the fields of plasmonics, fluorescence, and sensors. In an effort to better understand the influence of grating profile and metal film properties on the extraordinary fluorescence enhancement capabilities of plasmonic gratings, a novel microcontact printing process and different metal deposition techniques were used to fabricate silver gratings with varying grain diameters, roughnesses, heights, and duty cycles using thermal evaporation and RF sputtering. The resulting plasmonic gratings exhibited fluorescence enhancements up to 116x that of dye-coated glass slides using an epifluorescence microscope, much higher than more expensive prism-based SPR systems. This silver grating represents an extraordinary opportunity to quickly and easily enhance fluorescence and widen the detection limits of common fluorescence based assays with little to no equipment modification.
机译：自氟汞发动机的发展以来，研究人员寻求一种基本上提高荧光强度的方法，以延长对新敏感性水平的检测限。表面等离子体共振（SPR）和金属增强荧光长期以来一直是研究的主题，并导致了基于棱镜和光栅的SPR系统的开发。然而，利用简单的显微镜物镜具有宽耦合范围和易于激励SPR的令人兴奋的SPR，它们具有巨大的潜力，可彻底改变旋转野生物，荧光和传感器的田地。为了更好地了解光栅型材和金属膜性能对等离子体光栅的非凡荧光增强能力的影响，使用新的微接触印刷方法和不同的金属沉积技术来制造具有不同粒径，粗糙度，高度的银色光栅，使用热蒸发和射频溅射和占空比。所得到的等离子体光栅表现出使用离源性显微镜的染色玻璃载玻片的荧光增强，远高于更昂贵的棱镜的SPR系统。这款银光栅代表了快速，易于增强荧光的特殊机会，扩大了常见的荧光的检测限，几乎没有设备改性。

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  • 来源
    《RSC Advances》 |2015年第96期|共11页
  • 作者

    Wood A. J.; Chen B.; Pathan S.; Bok S.; Mathai C. J.; Gangopadhyay K.; Grant S. A.; Gangopadhyay S.;

  • 作者单位

    Univ Missouri Dept Bioengn Agr Engn 254 Columbia MO 65211 USA;

    Univ Missouri Dept Elect &

    Comp Engn Columbia MO 65211 USA;

    Univ Missouri Dept Elect &

    Comp Engn Columbia MO 65211 USA;

    Univ Missouri Dept Elect &

    Comp Engn Columbia MO 65211 USA;

    Univ Missouri Dept Elect &

    Comp Engn Columbia MO 65211 USA;

    Univ Missouri Dept Elect &

    Comp Engn Columbia MO 65211 USA;

    Univ Missouri Dept Bioengn Agr Engn 254 Columbia MO 65211 USA;

    Univ Missouri Dept Elect &

    Comp Engn Columbia MO 65211 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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