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Enhanced hydrogen production by photofermentative microbial aggregation induced by L-cysteine: the effect of substrate concentration, C/N ratio and agitation rate

机译:通过L-半胱氨酸诱导的光专用微生物聚集的增强氢产生:底物浓度,C / N比和搅拌速率的影响

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摘要

Continuous biomass washout from photobioreactors resulting from the poor flocculation of photofermentative bacteria (PFB) diverts electron flux away from hydrogen production to continuing biosynthesis. This work showed that three key parameters, the substrate concentration, C/N ratio and agitation rate significantly influenced the flocculation behavior of Rhodopseudomonas faecalis RLD-53. Excessive substrate (75 mmol l ~(-1) ) led an apparent drop in the flocculability, while a proper content of extracellular polymeric substances (EPS) at a C/N ratio of 10 : 2 was beneficial to aggregation of R. faecalis RLD-53. Control of the appropriate agitation rate not only enhanced EPS production and aggregation of PFB, but also avoided disruption of PFB aggregation caused by intensive hydrodynamic shear forces generated from excessive agitation. The optimal flocculability (47.67%) of R. faecalis RLD-53 was obtained at a substrate concentration of 50 mmol l ~(-1) , C/N ratio of 10 : 2 and agitation rate of 80 rpm. In the entire continuous operation, most PFB aggregates accumulated in the photobioreactor with only 0.55 g of biomass washout, much less than 2.86 g from free cell culture. Consequently, the maximum hydrogen yield of 2.57 mol H2 per mol acetate and production rate of 706 ml l ~(-1) d~(-1) were obtained by PFB aggregates, as biomass synthesis was greatly reduced. Therefore, aggregation of PFB is a promising strategy to mediate the reductant away from biomass synthesis into hydrogen production in continuous operation.
机译:从光学絮凝剂(PFB)的较差絮凝剂(PFB)转移到氢气产生至持续生物合成的情况下,从光生物测量器中脱离生物质冲洗。这项工作表明,三个关键参数,底物浓度,C / N比和搅拌速率显着影响了罗地孔的絮凝行为FAECALIS RLD-53。过量的基材(75mmol L〜(-1))LED在絮凝性中表现明显下降,而C / N比的细胞外聚合物物质(EPS)的适当含量有利于R. FAECALIS RLD的聚集-53。控制适当的激动速率不仅增强了PFB的EPS生产和聚集,还避免了由过度搅动产生的强烈流体动力剪切力引起的PFB聚集的破坏。在50mmol L〜(-1),C / N比为10:2的底物浓度下获得最佳絮凝性(47.67%)R. Faecalis RLD-53的含量,C / N比和80rpm的搅拌速率。在整个连续操作中,大多数PFB聚集体积聚在光生物反应器中,只有0.55g生物质冲洗,远低于自由细胞培养物。因此,通过PFB聚集体获得每摩尔乙酸盐的2.57mol H 2的最大氢产率和706ml L〜(-1)D〜(-1)的生产速率,因为生物量合成大大降低。因此,PFB的聚合是一种有希望的策略,用于将还原剂从生物量合成中介导在连续操作中的氢气产生。

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  • 来源
    《RSC Advances》 |2015年第111期|共7页
  • 作者单位

    State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China.;

    State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China.;

    State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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