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首页> 外文期刊>RSC Advances >Optimization and partial purification of a high-activity lipase synthesized by a newly isolated Acinetobacter from offshore waters of the Caspian Sea under solid-state fermentation
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Optimization and partial purification of a high-activity lipase synthesized by a newly isolated Acinetobacter from offshore waters of the Caspian Sea under solid-state fermentation

机译：在固态发酵下，在海洋海洋海外海外水分中新分离的分离的脂肪酶合成的高活性脂肪酶的优化和部分纯化

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摘要

A new aerobic mesophilic bacterium was isolated from the southern coastal waters of the Caspian Sea which substantially produced an extracellular lipase in solid-state fermentation using milled coriander seeds (MCS) as support substrate. This bacterium was identified as a strain of genus Acinetobacter based on morphological and biochemical characterization and 16S rRNA gene sequence. The various medium components and culture parameters to achieve a more cost effective and economically viable bioprocess were screened and optimized using the Plackett-Burman and central composite designs. The highest lipase activity (20 480.2 U g(-1)) was achieved at optimum levels of predominant factors of MCS/yeast extract (4.0 w/w), olive oil concentration (30 g L-1), moisture content (65.0%), and agitation rate (180.0 rpm). The enzyme with molecular weight of 46 kDa was purified 26.9-fold to homogeneity by ammonium sulfate precipitation and phenyl-Sepharose hydrophobic interaction chromatography. The functional groups of the lipase were also assigned using Fourier transform-infrared spectroscopy.
机译：从里海海洋的南部沿海水中分离出一种新的有氧嗜苯胺细菌，其在使用研磨的香菜种子（MCS）作为支撑基材的固态发酵中基本上产生了细胞外脂肪酶。基于形态学和生物化学表征和16S rRNA基因序列，将该细菌鉴定为异丙杆菌属的菌株。使用Plackett-Burman和中央复合设计来筛选和优化各种介质组件和培养参数，以实现更具成本效益和经济可行的生物过程。最高脂肪酶活性（20 480.2ug（-1））在MCS /酵母提取物（4.0W / W），橄榄油浓度（30g L-1），水分含量（65.0％）的最佳因子的最佳因子下实现）和搅拌率（180.0 rpm）。通过硫酸铵沉淀和苯琼脂糖疏水相互作用色谱法纯化具有46kDa的分子量为46kDa的酶至均匀性。还使用傅里叶变换红外光谱分配脂肪酶的官能团。

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  • 来源
    《RSC Advances》 |2015年第16期|共10页
  • 作者

    Moradi Samira; Razavi Seyed Hadi; Mousavi Seyyed Mohammad; Gharibzahedi Seyed Mohammad Taghi;

  • 作者单位

    Tech Univ BPEL Dept Food Sci Engn &

    Technol Fac Agr Engn &

    Technol Karaj 3158777871 Iran;

    Tech Univ BPEL Dept Food Sci Engn &

    Technol Fac Agr Engn &

    Technol Karaj 3158777871 Iran;

    Tarbiat Modares Univ Biotechnol Grp Dept Chem Engn Fac Engn Tehran Iran;

    Tech Univ BPEL Dept Food Sci Engn &

    Technol Fac Agr Engn &

    Technol Karaj 3158777871 Iran;

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