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首页> 外文期刊>RSC Advances >Metabolic regulation of alpha-linolenic acid on beta-carotene synthesis in Blakeslea trispora revealed by a GC-MS-based metabolomic approach
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Metabolic regulation of alpha-linolenic acid on beta-carotene synthesis in Blakeslea trispora revealed by a GC-MS-based metabolomic approach

机译：基于GC-MS基代代谢培养的Blakeslea Trisporaβ-胡萝卜素合成的α-亚麻酸的代谢调节

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摘要

Alpha-linolenic acid (ALA) is known for its ability to promote the production of beta-carotene in Blakeslea trispora. However, the mechanism is still poorly understood. In this study, gas chromatography-mass spectrometry (GC-MS)-based metabolomic approach and multivariate analysis were used to study mechanisms underlying the regulation effects of ALA on beta-carotene synthesis in B. trispora. ALA treatment promoted the biomass of B. trispora and beta-carotene production. The maximum beta-carotene production 5.344 mg L-1 was realized after 72 h of cultivation in the presence of 50 mu L ALA. The intracellular metabolite profiles found upon treatment of the cells with different addition time points of ALA were unique and could be distinguished from the aid of principal component analysis (PCA). Furthermore, partial least-squares-discriminant analysis (PLS-DA) revealed a group classification and pairwise discrimination between the control and ALA treated groups, and 28 differential metabolites with variable importance in the projection (VIP) value greater than 1. The addition of ALA decreased the glycolysis, TCA cycle and fatty acid synthesis. The accumulation of linolenic acid and linoleic acid indicated that ALA was directly absorbed by the fungus and transformed into its own linolenic acid. As a result, the flux from acetyl-CoA to beta-carotene synthesis increased. Besides, the addition of ALA increased the level of dissolved oxygen and the production of beta-carotene.
机译：已知α-亚麻酸（ALA）以其在Blowslea Trispora促进β-胡萝卜素的生产能力。然而，该机制仍然很清楚。在该研究中，使用气相色谱 - 质谱（GC-MS）基代的代谢物方法和多变量分析用于研究ALA对B. Trispora的β-胡萝卜素合成的调控作用的机制。 Ala治疗促进了B. trispora和β-胡萝卜素的生物量。在50亩LALA存在下72小时后，最大β-胡萝卜素生产5.344mg L-1。用不同的ALA的不同添加时间点治疗细胞发现的细胞内代谢物谱是独特的，并且可以从主成分分析（PCA）的辅助方案来区分。此外，局部最小二乘判别分析（PLS-DA）揭示了对照和ALA处理基团之间的组分类和成对判断，以及在投影中具有可变重要性的28个差分代谢物（VIP）值大于1.添加ALA降低了糖酵解，TCA循环和脂肪酸合成。亚麻酸和亚油酸的积累表明ALA被真菌直接吸收并转化为其自身的亚麻酸。结果，来自乙酰-CoA至β-胡萝卜素合成的助焊剂增加。此外，添加ALA增加了溶解氧水平和β-胡萝卜素的产生。

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  • 来源
    《RSC Advances》 |2015年第78期|共9页
  • 作者

    Hu Jing; Li Hao; Yang Yumeng; Wang Shizeng; Tang Pingwah; Li Chunfang; Tian Guifang; Yuan Qipeng;

  • 作者单位

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Ind Tech Coll Beijing 100023 Peoples R China;

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol Coll Life Sci &

    Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

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