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首页> 外文期刊>RSC Advances >Effect of high molecular weight hyaluronic acid on chondrocytes cultured in collagen/hyaluronic acid porous scaffolds
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Effect of high molecular weight hyaluronic acid on chondrocytes cultured in collagen/hyaluronic acid porous scaffolds

机译：高分子量透明质酸对胶原/透明质酸多孔支架培养的软骨细胞的影响

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摘要

Engineering cartilage tissue by culturing chondrocytes in porous scaffolds is one promising method to repair or restore the functions of diseased cartilage. Hyaluronic aid (HA) was used in porous scaffolds or hydrogels to promote the proliferation of chondrocytes and synthesis of cartilage extracellular matrix (ECM). However, whether HA in scaffolds has a beneficial effect on chondrocytes remains uncertain, possibly due to the uncontrolled pore structure and inhomogeneous HA in scaffolds. In this study, homogeneous collagen/HA scaffolds with well-controlled and interconnected pore structure were prepared by suppression of polyion complex formation between collagen and HA and using ice particulates as porogen. The pore structure and mechanical property of collagen/HA scaffolds and collagen scaffolds could be well controlled. High molecular weight HA in collagen/HA scaffolds inhibited the cellular proliferation, synthesis of sulfated glycosaminoglycan and cartilage ECM, compared with the results of collagen scaffolds. This study should provide additional information on the effects of HA in porous scaffolds on the chondrocyte behaviour in 3D culture.
机译：通过培养多孔支架中的软骨细胞来修复或恢复患病软骨的功能的一种有望的方法。透明质病助剂（HA）用于多孔支架或水凝胶，以促进软骨细胞的增殖和软骨细胞外基质（ECM）的合成。然而，在支架中的HA是否对软骨细胞有益效果仍然不确定，可能是由于由于不受控制的孔结构和支架中的不均匀HA。在该研究中，通过抑制胶原和HA之间的聚硫末络合物形成并使用冰颗粒作为孔剂来制备具有良好控制和相互连接的孔结构的均匀胶原/ HA支架。胶原蛋白/ HA支架和胶原屑支架的孔结构和机械性能可以很好地控制。与胶原支架的结果相比，胶原/ HA支架中的高分子量HA抑制了细胞增殖，合成硫酸化糖胺聚糖和软骨ECM。本研究应提供有关HA在多孔支架上对3D培养中软骨细胞行为的影响的额外信息。

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  • 来源
    《RSC Advances》 |2015年第114期|共6页
  • 作者

    Shangwu Chen; Qin Zhang; Naoki Kawazoe;

  • 作者单位

    Tissue Regeneration Materials Unit International Center for Materials Nanoarchitectonics National Institute for Materials Science 1-1 Namiki Tsukuba Ibaraki 305-0044 Japan.;

    Tissue Regeneration Materials Unit International Center for Materials Nanoarchitectonics National Institute for Materials Science 1-1 Namiki Tsukuba Ibaraki 305-0044 Japan.;

    Department of Materials Science and Engineering Graduate School of Pure and Applied Sciences University of Tsukuba 1-1-1 Tennodai Tsukuba Ibaraki 305- 8571 Japan;

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  • 正文语种 eng
  • 中图分类 化学;
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