• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Performance in synthetic applications of a yeast surface display-based biocatalyst
【24h】

Performance in synthetic applications of a yeast surface display-based biocatalyst

机译：基于酵母表面显示的生物催化剂的合成应用性能

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This work demonstrates the effectiveness of yeast surface display (YSD) as a scaffold for biocatalysts in hydrophobic, non-aqueous environments. Two lipases, Candida antarctica lipase B (CalB) and Photobacterium lipolyticum sp. M37 lipase (M37L), were immobilized independently by surface display on Saccharomyces cerevisiae. The two YSD biocatalysts were employed to synthesize esters of butanol and saturated fatty acids of varying length (8 to 16 carbons) in heptane. Effects of fatty acid chain length and temperature on the esterification reaction were examined. The YSD catalysts synthesized butyl decanoate in 10 repeated batches with little loss in activity. Compared to a commercial immobilized lipase (Novozym 435), the activity of both YSD lipases was lower on a mass loading basis, but higher when normalized on estimates of protein loading. Initial-rate kinetics of the butyl decanoate reaction were measured for the CalB-displaying yeast. Kinetics and apparent activity of M37L in the multi-batch experiments depend heavily on water concentration; kinetics for M37L could not be elucidated with initial-rate methods. The difference between CalB and M37L in water requirements illustrates a critical parameter for optimization of lipase activity in non-aqueous environments. The activity of both lipases in a completely hydrophobic environment is a step towards more economical biocatalysis of industrial esterification.
机译：这项工作证明了酵母表面显示（YSD）作为疏水性，非水环境中的生物催化剂的支架的有效性。两种脂肪酶，念珠菌抗原脂肪酶B（CALB）和Photobacterium Lipolyticum sp。 M37脂肪酶（M37L），通过表面显示在酿酒酵母上独立地固定。使用两种YSD生物催化剂在庚烷中合成不同长度（8至16碳）的丁醇和饱和脂肪酸的酯。检查脂肪酸链长度和温度对酯化反应的影响。 YSD催化剂在10重复批次中合成丁基丁酯，活性少量损失。与商业固定化脂肪酶（Novozym 435）相比，ysd脂肪酶的活性在质量加载的基础上较低，但是当蛋白质载荷估计上归一化时更高。测量癸酸丁酯反应的初始速率动力学对CALB显示酵母进行测量。 M37L在多批次实验中的动力学和明显活性依赖于水浓度; M37L的动力学不能用初始速率方法阐明。 CALB和M37L在水要求之间的差异说明了用于优化非水环境中的脂肪酶活性的关键参数。两种脂肪酶在完全疏水的环境中的活性是朝向更经济的工业酯化生物分析的一步。

著录项

  • 来源
    《RSC Advances》 |2015年第39期|共8页
  • 作者

    Eby J. M.; Peretti S. W.;

  • 作者单位

    N Carolina State Univ Dept Chem &

    Biomol Engn Raleigh NC 27695 USA;

    N Carolina State Univ Dept Chem &

    Biomol Engn Raleigh NC 27695 USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号