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首页> 外文期刊>RSC Advances >Surfactant induced aggregation-disaggregation of photodynamic active chlorin e6 and its relevant interaction with DNA alkylating quinone in a biomimic micellar microenvironment
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Surfactant induced aggregation-disaggregation of photodynamic active chlorin e6 and its relevant interaction with DNA alkylating quinone in a biomimic micellar microenvironment

机译：表面活性剂诱导光动力活性氯蛋白E6的聚集分解及其与Biomimic胶束微环境中DNA烷基化醌的相关相互作用

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摘要

The present paper epitomizes the detailed physicochemical behaviour of photodynamic active chlorin e6 (Ce6) and its interaction with the DNA alkylating quinone; 2,5-dichloro diaziridinyl-1,4-benzoquinone (AZDCIQ) in different bio-mimicking micellar microenvironments using steady state absorption, fluorescence, time resolved fluorescence and fluorescence anisotropy studies. Dramatic modulation in the photophysics of Ce6 has been observed in two types of surfactant assemblies namely premicellar and postmicellar assemblies of cationic CTAB, anionic SDS and nonionic TX-100. In water at pH 7.4, Ce6 exists as monomeric (73%, 4.91 ns) and dimeric (27%, 2.49 ns) forms, but with increasing concentration of CTAB in the premicellar region, the absorption and emission intensity decreases significantly due to the formation of surfactant induced higher aggregates. Interestingly further addition of CTAB (at critical micellar concentration and above) leads to disaggregation of those higher aggregates into its subsequent monomeric and dimeric form. In the case of TX-100 and SDS, the dye does not form higher aggregates in the premicellar region, rather it remains as monomeric-dimeric forms throughout the concentration range until the critical micellar concentration (CMC). After micellization, the percentage of Ce6 monomer increases in the case of TX-100, whereas the reverse case is observed in SDS, which may be explained by the forced dimerisation caused by repulsive interactions between anionic Ce6 and SDS micelles. The copper induced fluorescence quenching, solvation dynamics, and fluorescence anisotropy shows that the dye is localised in the Stern layer of CTAB, in the palisade layer of TX-100 and in the outer Gouy-Chapman layer or in aqueous bulk phase in the case of SDS micelles. The interaction of fully micellized PDT active Ce6 with DNA alkylating AZDCIQ is found to be more in CTAB as compared to TX-100 and water. The solvation dynamics of Ce6 in the presence of the quinone reveals the dynamic nature of the interaction between these two partners. The spectroscopic research described herein may provide numerous effective information for the use of chlorin(s) and alkylating quinones together to overcome the limitation of PDT, especially in the hypoxic environment of solid tumors.
机译：本论文集中体现光动力活性二氢卟酚e6（的Ce6）的详细物理化学行为以及其与DNA烷化剂醌相互作用; 2,5-二氯在使用稳定状态的吸收，荧光，时间分辨荧光和荧光各向异性研究不同生物模仿胶束微环境diaziridinyl -1,4-苯醌（AZDCIQ）。在的Ce6的光物理戏剧性调制已在两种类型的表面活性剂组件即premicellar和阳离子CTAB的postmicellar组件，阴离子SDS和非离子TX-100的被观察到。在pH 7.4的水，的Ce6存在作为单体（73％，4.91 NS）和二聚体（27％，2.49纳秒）的形式，但是随着premicellar区域增加CTAB的浓度，吸收和发射强度由于形成显著降低表面活性剂的诱导更高的聚集体。有趣的是进一步添加CTAB的（在临界胶束浓度以上）导致更高的那些聚集体的解聚成其随后的单体和二聚体形式。在TX-100和SDS的情况下，染料不在premicellar区域形成更高的聚集体，而它仍然在整个浓度范围内的单体，二聚体形式，直到临界胶束浓度（CMC）。胶束后，在TX-100的情况下的Ce6单体的百分比增加，而相反的情况下在SDS观察到，这可能是由阴离子的​​Ce6和SDS胶束之间排斥相互作用的强制二聚化来解释。铜诱导的荧光猝灭，溶剂化动力学，和荧光各向异性表明，染料在CTAB的斯特恩层局部的，在TX-100的栅栏层和在外部的Gouy-查普曼层或在含水体相的情况下SDS胶束。的充分胶束化PDT活性的Ce6用DNA烷基化AZDCIQ的相互作用被认为是更CTAB比TX-100和水。的Ce6在醌的存在下溶剂化动力学揭示了这两个伙伴之间的相互作用的动态性质。分光研究本文所述的可提供用于使用的二氢卟酚的（一个或多个）和烷基化醌一起克服PDT的限制，特别是在实体肿瘤的低氧环境许多有效的信息。

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  • 来源
    《RSC Advances》 |2015年第99期|共12页
  • 作者

    Jadhao Manojkumar; Ahirkar Piyush; Kumar Himank; Joshi Ritika; Meitei Oinam Romesh; Ghosh Sujit Kumar;

  • 作者单位

    Visvesvaraya Natl Inst Technol Dept Chem Nagpur 440010 Maharashtra India;

    Visvesvaraya Natl Inst Technol Dept Chem Nagpur 440010 Maharashtra India;

    Visvesvaraya Natl Inst Technol Dept Chem Nagpur 440010 Maharashtra India;

    Visvesvaraya Natl Inst Technol Dept Chem Nagpur 440010 Maharashtra India;

    Visvesvaraya Natl Inst Technol Dept Chem Nagpur 440010 Maharashtra India;

    Visvesvaraya Natl Inst Technol Dept Chem Nagpur 440010 Maharashtra India;

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  • 正文语种 eng
  • 中图分类 化学;
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