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Dispersion of multiwalled carbon nanotubes in Acacia extract and it's utility as an antimicrobial agent

机译:含有多壁碳纳米管在抗菌剂中的多壁碳纳米管分散

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摘要

This work deals with the preparation of a multiwalled carbon nanotubes (MWCNTs) dispersion in Acacia extract (AE) and checks its antimicrobial activity. UV-vis analysis, Fourier transform infra-red spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to confirm the surface adsorption of extract components on MWCNT. The dynamic light scattering (DLS) determined the zeta potential and the particle size distribution (PSD) of MWCNTs in the AE dispersion medium. To observe the MWCNT stability and dispersion in AE, Turbiscan and transmission electron microscopy (TEM) were used respectively. The MWCNTs were found dispersed till the end of day 30, which suggests that AE is a good dispersing medium due to its surfactant properties. Finally, the dispersion was tested for its applicability as an antimicrobial agent using E. coli as a model microorganism. Colony forming units (CFUs) and dehydrogenase activity were assessed to check the inhibitory effect of dispersion. To test cell damage, fluorescent-based assay was conducted. The reduction in the viable bacterial count in the test sample proved the ability of dispersion as an antimicrobial agent. We are the first to report that AE may provide an alternative to the chemical surfactant for MWCNTs dispersion, and the AE-MWCNTs dispersion can be utilized as an antimicrobial agent.
机译:这项工作在合欢提取物(AE),并检查其抗微生物活性的多壁碳纳米管(MWCNT)分散液的制备涉及。的UV-vis分析,傅里叶变换红外光谱(FT-IR)和扫描电子显微镜(SEM)被用来确认对MWCNT提取物组分的表面吸附。动态光散射(DLS)确定的ζ电势和在AE分散介质多壁碳纳米管的粒度分布(PSD)。观察AE,Turbiscan和透射电子显微镜(TEM)的MWCNT稳定性和分散分别使用。发现多壁碳纳米管分散直到第30天,这表明AE是一个很好的分散介质的端部,由于其表面活性剂性能。最后,将分散体对于其适用性如使用大肠杆菌作为模型微生物的抗微生物剂进行测试。菌落形成单位(CFU)和脱氢酶活性进行了评估,以检查分散体的抑制作用。为了测试细胞损伤,基于荧光的测定法进行的。在测试样品中的活菌数的减少证明了分散体作为抗微生物剂的能力。我们是第一个报告,AE可以提供对化学的表面活性剂为多壁碳纳米管分散液替代,以及AE-多壁碳纳米管分散液可以用作抗微生物剂。

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  • 来源
    《RSC Advances》 |2015年第126期|共8页
  • 作者单位

    Sardar Vallabhbhai Natl Inst Technol Dept Chem Engn Surat 395007 Gujarat India;

    Sardar Vallabhbhai Natl Inst Technol Dept Chem Engn Surat 395007 Gujarat India;

    Sardar Vallabhbhai Natl Inst Technol Dept Chem Engn Surat 395007 Gujarat India;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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