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首页> 外文期刊>RSC Advances >Piperine promotes ultraviolet (UV)-B-induced cell death in B16F10 mouse melanoma cells through modulation of major regulators of cell survival
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Piperine promotes ultraviolet (UV)-B-induced cell death in B16F10 mouse melanoma cells through modulation of major regulators of cell survival

机译：哌啶通过调制细胞存活的主要调节剂促进B16F10小鼠黑素瘤细胞中的紫外线（UV）-B诱导的细胞死亡

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摘要

An increase in the incidence of melanoma and its resistance to currently applied treatment regimes enhance the need for novel therapeutic agents and treatment modalities. In this study, we report that piperine, the most widely consumed dietary alkaloid, promotes cell death in ultraviolet (UV)-B-irradiated B16F10 mouse melanoma cells through the elevation of intracellular reactive oxygen species (ROS) formation, calcium homeostasis imbalance, and loss of mitochondrial membrane potential. Promotion of UVB-induced cell death by piperine was further revealed by caspase activations, poly(ADP) ribose polymerase cleavage, DNA fragmentation, and an increase in sub-G1 cells. Piperine promoted UVB-induced translocation of Bax from cytosol to mitochondria, accelerated an increase in the ratio of Bax to that of Bcl-2, and up-regulated the expression of apoptosis-inducing factor (AIF). These effects of piperine on UVB-irradiated cells were associated with apparent alterations in the expression of mitogen activated protein (MAP) kinase family proteins and PI3K-Akt survival signals. Piperine disrupted NF-kappa B signaling, inhibited UVB-induced nuclear translocation of NF-kappa B, and potentially reversed multi drug resistance (MDR) by reducing UVB-induced p-glycoprotein activity. Taken together, these results suggest the possibility of using piperine in combination with UVB as a possible therapeutic option for melanoma.
机译：在黑色素瘤的发病率及其对当前应用的治疗方案电阻的增加提高了新的治疗剂和治疗方法的需要。在这项研究中，我们报告，胡椒碱，最广泛饮用的饮食生物碱，促进紫外线（UV）-B照射B16F10小鼠黑色素瘤细胞的细胞死亡通过细胞内活性氧簇（ROS）的形成，钙稳态失调的标高，并线粒体膜电位的损失。胡椒碱UVB诱导的细胞死亡的促进通过胱天蛋白酶激活，聚（ADP）核糖聚合酶裂解，DNA片段化，并增加了亚G1细胞中进一步揭示。胡椒碱促进从细胞溶质Bax蛋白的UVB诱导易位至线粒体，加速Bax蛋白到的Bcl-2的比率的增加，并且上调的凋亡诱导因子（AIF）的表达。胡椒碱对UVB照射的细胞中的这些作用与表观改变在丝裂原活化蛋白（MAP）激酶家族蛋白和PI3K-AKT存活信号的表达相关联。胡椒碱破坏NF-κB信号，NF-κB的抑制的UVB诱导的核易位，并且潜在地减少UVB诱导的p-糖蛋白活性的逆转多重抗药性（MDR）。两者合计，这些结果表明在组合使用胡椒碱与UVB作为黑色素瘤的可能的治疗选择的可能性。

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  • 来源
    《RSC Advances》 |2015年第16期|共11页
  • 作者

    Rafiq Rather A.; Ganai Bashir A.; Tasduq Sheikh A.;

  • 作者单位

    Indian Inst Integrat Med CSIR PK PD &

    Toxicol Div Jammu Jammu &

    Kashmir India;

    Univ Kashmir Ctr Res Dev CORD Srinagar 190006 Jammu &

    Kashmir India;

    Indian Inst Integrat Med CSIR PK PD &

    Toxicol Div Jammu Jammu &

    Kashmir India;

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  • 正文语种 eng
  • 中图分类 化学;
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