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首页> 外文期刊>RSC Advances >Rapid and high-density covalent immobilization of Rhizomucor miehei lipase using a multi component reaction: application in biodiesel production
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Rapid and high-density covalent immobilization of Rhizomucor miehei lipase using a multi component reaction: application in biodiesel production

机译：使用多组分反应的Rhizomucor Miehei脂肪酶快速和高密度共价固定：在生物柴油生产中的应用

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摘要

Aldehyde-functionalized silica and silica nanoparticles (SBA-15) were prepared as a matrix system for enzyme immobilization. Immobilization of Rhizomucor miehei lipase (RML) on these supports was performed via a multicomponent reaction under extremely mild conditions (25 degrees C, pH 7). Investigation on the mechanism of this reaction confirmed the Ugi four-component immobilization approach. The loading capacity of the supports and specific activity of the immobilized derivatives were interestingly improved. The results revealed very rapid immobilization of 10 and 60 mg of RML on 1 g of aldehyde-functionalized silica and SBA-15 after 10 and 30 minutes, respectively. Leaching experiments were performed by incubation of the immobilized derivatives in 1 M NaCl solution. The lack of the free lipase in the solution confirmed the covalent nature of the linkage. The thermal stability and co-solvent stability of the derivatives in the presence of three polar organic solvents (1-propanol, 2-propanol and dioxane) were greatly improved compared to the soluble enzyme. Both the derivatives were also used to catalyze the transesterification of colza oil with methanol to produce fatty acid methyl esters (FAMEs). In the case of RML immobilized on SBA-15 (SBA-RML), the presence of 40% of tert-butanol (v/v) as solvent in the reaction medium largely improved the conversion yield.
机译：制备醛官能化二氧化硅和二氧化硅纳米颗粒（SBA-15）作为酶固定化的基质系统。通过极温度的条件（25℃，pH 7），通过多组分反应进行这些载体上的根瘤菌脂肪酶（RM1）的固定化。对该反应机制的研究证实了UGI四组分固定方法。载体的负载和固定衍生物的特定活动的装载能力有趣地改善。结果表明，在10至30分钟后，在1g醛官能化二氧化硅和SBA-15中非常快速地固定10和60mg RML。通过在1M NaCl溶液中孵育固定的衍生物进行浸出实验。溶液中缺乏自由脂肪酶证实了连锁的共价性质。与可溶性酶相比，大大提高了三种极性有机溶剂（1-丙醇，2-丙醇和二恶烷）存在下衍生物的热稳定性和共溶剂稳定性。衍生物也用于催化大肠杆菌与甲醇的酯交换，以生产脂肪酸甲酯（FAME）。在固定在SBA-15（SBA-RML）上的RML的情况下，在反应介质中存在40％的叔丁醇（v / v），在反应介质中的溶剂大大提高了转化率。

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