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首页> 外文期刊>RSC Advances >AFM and NMR imaging of squid tropomyosin Tod p1 subjected to high hydrostatic pressure: evidence for relationships among topography, characteristic domain and allergenicity
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AFM and NMR imaging of squid tropomyosin Tod p1 subjected to high hydrostatic pressure: evidence for relationships among topography, characteristic domain and allergenicity

机译：对鱿鱼的鱿鱼Tropomyosin TOD P1进行AFM和NMR成像经受高静水压压力：地形，特征结构域和过敏性关系的证据

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摘要

The surface topography, characteristic domain and allergenicity of squid tropomyosin Tod p1 (TMTp1) treated under single- and two-cycle high hydrostatic pressure (HHP) were analyzed. Atomic force microscopy (AFM) showed that the HHP treatment led to the formation of a rougher surface of squid TMTp1; the two-cycle 600 MPa HHP treatment produced the largest effect, with a mean roughness, maximum height, skewness and kurtosis of 6.56 nm, 94.83 nm, 3.05 and 23.13, respectively. The HHP treatment caused lower IgE and IgG-binding capacities, indicating a significant reduction of the allergenicity (p < 0.05) due to variations in the AFM surface topography. The peaks of the main allergenic characteristics affected were 0.99, 1.16, 1.21, 1.79, 1.82, 2.84, 2.88 and 3.37 ppm (in the initial H-1 nuclear magnetic resonance (NMR) spectra). The HHP treatment changed the AFM surface topography and caused the migration or disappearance of the related H-1 NMR peaks; these changes were significantly correlated (p < 0.05) with the reductions in allergenicity observed.
机译：分析了在单周期和双循环高静液压（HHP）下处理的鱿鱼番茄素TOD P1（TMTP1）的表面形貌，特征结构域和过敏性。原子力显微镜（AFM）显示HHP处理导致形成鱿鱼TMTP1的粗糙表面;双周期600MPa HHP处理产生了最大的效果，分别具有6.56nm，94.83nm，3.05和23.13的平均粗糙度，最大高度，偏纹和峰值。 HHP处理引起了降低的IgE和IgG结合能力，表明由于AFM表面形貌的变化，过敏性的显着降低了（P <0.05）。受影响的主要过敏性特性的峰为0.99,1.16,1.21,1.79,1.82,2.84,2.88和3.37ppm（在初始H-1核磁共振（NMR）光谱中）。 HHP治疗改变了AFM表面形貌并导致相关H-1 NMR峰的迁移或消失;这些变化显着相关（P <0.05）随着偏移的减少观察到。

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  • 来源
    《RSC Advances》 |2015年第89期|共10页
  • 作者

    Zhang Yifeng; Dai Bona; Deng Yun; Zhao Yanyun;

  • 作者单位

    Shanghai Jiao Tong Univ Dept Food Sci &

    Technol Key Lab Urban Agr South Minist Agr SJTU Bor S Luh Food Safety Ctr Shanghai 200240 Peoples R China;

    Shanghai Jiao Tong Univ Instrumental Anal Ctr Shanghai 200240 Peoples R China;

    Shanghai Jiao Tong Univ Dept Food Sci &

    Technol Key Lab Urban Agr South Minist Agr SJTU Bor S Luh Food Safety Ctr Shanghai 200240 Peoples R China;

    Oregon State Univ Dept Food Sci &

    Technol Corvallis OR 97331 USA;

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  • 正文语种 eng
  • 中图分类 化学;
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