A simple and reversible fluorescent probe based on NBD for rapid detection of hypochlorite and its application for bioimaging

Shen Youming; Zhang Xiangyang; Huang Xi; Wen Siyu; Liu Mingdi; Deng Yan; Zhang Youyu; Zhang Chunxiang; Jin Junling; Li Haitao; Yao Shouzhuo

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A simple and reversible fluorescent probe based on NBD for rapid detection of hypochlorite and its application for bioimaging

机译：基于NBD的简单可逆荧光探针，用于快速检测次氯酸盐及其对生物体的应用

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摘要

A simple and reversible fluorescent probe bearing 7-nitrobenz-2-oxa-1,3-diazole and a selenomorpholine fragment was designed and synthesized. The probe showed highly selective, sensitive and fast (<10 s) recognition to hypochlorite in aqueous solutions. The relative results demonstrated that the linear response range of the probe was between 5.0 x 10(-8) M and 1.2 x 10(-4) M, with a low detection limit of 3.3 nM (S/N = 3). The probe was capable of monitoring hypochlorite reversibly in the presence of glutathione. In addition, the biological applications in living cells have been described.

机译：设计并合成了一种简单且可逆的荧光探针轴承7-NITROBENZ-2-OXA-1,3-二氮杂物和硒卟啉片段。探针显示出高度选择性，敏感和快速（<10秒）在水溶液中的次氯酸盐识别。相对结果表明探针的线性响应范围在5.0×10（ - 8）m和1.2×10（-4）m之间，检测限为3.3nm（s / n = 3）。探针在谷胱甘肽的存在下可逆地监测次氯酸盐。此外，已经描述了活细胞中的生物学应用。

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来源
《RSC Advances》 |2015年第97期|共6页
作者
Shen Youming; Zhang Xiangyang; Huang Xi; Wen Siyu; Liu Mingdi; Deng Yan; Zhang Youyu; Zhang Chunxiang; Jin Junling; Li Haitao; Yao Shouzhuo;
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作者单位

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Normal Univ Coll Chem &

Chem Engn Minist Educ Key Lab Chem Biol &

Tradit Chinese Med Res Changsha 410081 Hunan Peoples R China;

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Normal Univ Coll Chem &

Chem Engn Minist Educ Key Lab Chem Biol &

Tradit Chinese Med Res Changsha 410081 Hunan Peoples R China;

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Univ Arts &

Sci Coll Chem &

Chem Engn Changde 415000 Peoples R China;

Hunan Normal Univ Coll Chem &

Chem Engn Minist Educ Key Lab Chem Biol &

Tradit Chinese Med Res Changsha 410081 Hunan Peoples R China;

Hunan Normal Univ Coll Chem &

Chem Engn Minist Educ Key Lab Chem Biol &

Tradit Chinese Med Res Changsha 410081 Hunan Peoples R China;

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正文语种 eng
中图分类化学;
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1. A simple and reversible fluorescent probe based on NBD for rapid detection of hypochlorite and its application for bioimaging [J] . Shen Youming, Zhang Xiangyang, Huang Xi, RSC Advances . 2015,第97期

机译：基于NBD的简单可逆荧光探针，用于快速检测次氯酸盐及其对生物体的应用
2. A new red fluorescent probe based on rosamine-phenothiazine for the highly selective and rapid detection of hypochlorite and its bioimaging in live cells [J] . Zhao Yun, Xue Yuanyuan, Sun Juanjuan, New Journal of Chemistry . 2020,第29期

机译：一种基于玫瑰花 - 吩噻嗪的新的红荧光探针，用于高选择性和快速检测次氯酸盐及其在活细胞中的生物成像
3. A cyanobiphenyl based fluorescent probe for rapid and specific detection of hypochlorite and its bio-imaging applications [J] . Xu Tang, Zhi Zhu, Yun Wang, Sensors and Actuators . 2018,第JUNa期

机译：一种基于氰基联苯的荧光探针，可快速，特异性地检测次氯酸盐及其生物成像应用
4. A two-photon fluorescent probe for Cu2+based on dansyl chloride and its application in bioimaging [C] . Huie Jiang, Zhijian Li, Hui Zhu, 第五届制浆造纸新技术国际研讨会（ISETPP）暨第三届国际造纸与环境学术大会（IPEC）论文集 . 2016

机译：基于丹酰氯的Cu2 +的双光子荧光探针及其在生物成像中的应用
5. Small Molecule-based Fluorescent Molecular Probes for Facilitating Biomedical Research: Rational Design and Bioimaging Applications [D] . Yan, Xin. 2019

机译：用于促进生物医学研究的小分子荧光分子探针：理性设计和生物体分析应用
6. A Coumarin-Based Fluorescent Probe for Ratiometric Detection of Cu2+ and Its Application in Bioimaging [O] . Jie Zhang, Meng-Yu Chen, Cui-Bing Bai, 2020

机译：一种基于香豆素的荧光探针用于比例检测Cu2 +及其在BioMimaging中的应用
7. A Sensitive and Selective Fluorescent Coumarin-Based Probe for Detection of Hypochlorite ion and its Application to Cellular Imaging [O] . Hou, Rui-Chun, Ding, Xiang-Qian, Gong, Hui-Li, 2016

机译：灵敏的选择性香豆素荧光探针可检测次氯酸根离子及其在细胞成像中的应用

A simple and reversible fluorescent probe based on NBD for rapid detection of hypochlorite and its application for bioimaging

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