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首页> 外文期刊>RSC Advances >A rapid, sensitive and label-free sensor for Hg(II) ion detection based on blocking of cysteine-quenching of fluorescent poly(thymine)-templated copper nanoparticles
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A rapid, sensitive and label-free sensor for Hg(II) ion detection based on blocking of cysteine-quenching of fluorescent poly(thymine)-templated copper nanoparticles

机译：用于HG（II）离子检测的快速，灵敏和无标记的传感器，基于荧光聚（胸腺嘧啶）的半胱氨酸淬火阻断 - 铜纳米粒子

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摘要

A simple, rapid, sensitive and label-free fluorescence sensor has been developed for Hg(II) ion detection on the basis of blocking of cysteine-quenching of fluorescent poly(thymine) (poly T)-templated copper nanoparticles (Cu NPs). Fluorescent Cu NPs as fluorescence probe were formed within 5 minutes under mild conditions by using T30 DNA as template. In the presence of cysteine, the fluorescence intensity of T30-templated Cu NPs was found to be quenched effectively, due to the formation of a coordination complex by the Cu-S metal-ligand bond between the Cu NPs and the cysteine. However, when Hg~(2+) was added, the more stable Hg-S bond was formed through the strong binding preference of cysteine toward Hg~(2+), causing the cysteine to be far away from the surface of T30-templated fluorescent Cu NPs and hence the fluorescence intensity recovered. Under the optimized conditions, the sensor achieved highly sensitive and selective detection of Hg~(2+) in the range from 0.5 nM to 100 nM and the detection limit was 0.1 nM. Furthermore, the label-free method was successfully applied in the detection of Hg~(2+) in lake water samples. Besides, the developed sensor also showed high selectivity, low cost, and simplified operation. Thus, it would hold considerable potential to construct a simple, selective and sensitive fluorescence platform for the detection of Hg(II) ions.
机译：基于阻断荧光聚（胸腺嘧啶）（Poly T） - 铜纳米粒子（Cu NPS）的半胱氨酸猝灭，开发了一种简单，快速，敏感和无标记的荧光传感器的离子检测。通过使用T30 DNA作为模板，在温和条件下在5分钟内形成荧光Cu nps作为荧光探针。在半胱氨酸存在下，发现T30模板化Cu NPS的荧光强度有效地淬灭，由于Cu NPS与半胱氨酸之间的Cu-S金属 - 配体键形成配合复合物。然而，当加入Hg〜（2+）时，通过朝向Hg〜（2+）的致胱内半胱氨酸的强结合偏好形成更稳定的Hg-s键，导致半胱氨酸远离T30模板的表面很远荧光Cu nps并因此回收荧光强度。在优化条件下，传感器在0.5nm至100nm的范围内实现高敏感和选择性检测Hg〜（2+），检测限为0.1nm。此外，在湖水样品中的Hg〜（2+）检测中成功地应用了无标记方法。此外，发达的传感器还显示出高选择性，低成本和简化操作。因此，它将具有相当大的潜力来构建用于检测HG（II）离子的简单，选择性和敏感的荧光平台。

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  • 来源
    《RSC Advances》 |2015年第114期|共6页
  • 作者

    Hai-Bo Wang; Ying Chen; Yang Li;

  • 作者单位

    College of Chemistry and Chemical Engineering Xinyang Normal University Xinyang 464000 PR China.;

    College of Chemistry and Chemical Engineering Xinyang Normal University Xinyang 464000 PR China.;

    College of Chemistry and Chemical Engineering Xinyang Normal University Xinyang 464000 PR China.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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