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首页> 外文期刊>RSC Advances >Fabrication of AS1411 aptamer functionalized Gd2O3-based molecular magnetic resonance imaging (mMRI) nanoprobe for renal carcinoma cell imaging
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Fabrication of AS1411 aptamer functionalized Gd2O3-based molecular magnetic resonance imaging (mMRI) nanoprobe for renal carcinoma cell imaging

机译：AS1411适体官能化GD2O3的分子磁共振成像（MMRI）纳米孔的制备用于肾癌细胞成像

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摘要

Magnetic resonance imaging (MRI) as a noninvasive diagnostic technology with high spatial resolution has been widely used in clinics. However, the relatively low sensitivity is the main shortcoming of this technology. To address this issue, we would like to develop a molecular MRI nanoprobe for the sensitive and specific MRI of renal carcinoma cells with BSA-Gd2O3 nanoparticles as MRI contrast agents, mesoporous silica nanoparticles (mSiO(2) NPs) as nanocarriers and the AS1411 aptamer as a targeting molecule. To achieve this aim, BSA-Gd2O3 NPs were assembled onto mSiO(2) NPs with the help of anionic polyelectrolyte, sodium polystyrene sulfonate (PSS), and cationic polyelectrolyte, poly dimethyl diallyl ammonium chloride (PDDA) layer by layer. Such successful assembly was confirmed by transmission electron microscopy (TEM), FT-IR spectroscopy, zeta-potential analysis, hydrodynamic diameter determination and gel electrophoresis. After assembly, the mSiO(2)/PSS/PDDA/BSA-Gd2O3 nanoprobe presented a larger longitudinal relaxivity (r(1)) (26.1 s(-1) mM(-1) Gd) than BSA-Gd2O3 NPs (11.8 s(-1) mM(-1) Gd) and commercially used Gd-DTPA (3.87 s(-1) mM(-1) Gd). Additionally, with the AS1411 aptamer as a targeting molecule, our fabricated mSiO(2)/PSS/PDDA/BSA-Gd2O3-AS1411 nanoprobes could recognize clear cell renal carcinoma cells (ccRCC) specifically by MRI in vitro.
机译：磁共振成像（MRI）作为具有高空间分辨率的非侵入性诊断技术已被广泛用于诊所。然而，相对较低的灵敏度是这项技术的主要缺点。为了解决这个问题，我们希望用BSA-Gd2O3纳米粒子作为MRI造影剂，中孔二氧化硅纳米粒子（MSIO（2）NPS）作为纳米载体和AS1411适体的敏感剂和AS1411A适体的敏感剂和特异性MRI进行分子MRI Nanoprobe。作为靶向分子。为了实现这一目标，通过阴离子聚电解质，聚苯乙烯磺酸钠（PSS）和阳离子聚电解质，聚二甲基二烯丙基氯化铵（PDDA）层，将BSA-Gd2O3 NPS组装到MSIO（2）NPS上。通过透射电子显微镜（TEM），FT-IR光谱，Zeta-电位分析，流体动力学测定和凝胶电泳来确认这种成功的组装。组装后，MSIO（2）/ PSS / PDDA / BSA-GD2O3纳米孔呈较大的纵向松弛率（R（1））（26.1S（-1）mm（-1）gd）比BSA-GD2O3 NPS（11.8秒（-1）mm（-1）gd）和商业上使用的gd-dtpa（3.87 s（-1）mm（-1）gd）。另外，通过AS1411适体作为靶向分子，我们的制造的MSIO（2）/ PSS / PDDA / BSA-GD2O3-AS1411纳米体纳米体可以通过MRI体外识别透明细胞肾癌细胞（CCRCC）。

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  • 来源
    《RSC Advances》 |2015年第94期|共7页
  • 作者

    Dai Yue; Zhang Aiping; You Jia; Li Jingjing; Xu Huiting; Xu Kai;

  • 作者单位

    Xuzhou Med Coll Affiliated Hosp Dept Radiol Xuzhou 221006 Peoples R China;

    Xuzhou Med Coll Sch Med Imaging Xuzhou 221004 Peoples R China;

    Xuzhou Med Coll Sch Med Imaging Xuzhou 221004 Peoples R China;

    Xuzhou Med Coll Affiliated Hosp Dept Radiol Xuzhou 221006 Peoples R China;

    Xuzhou Med Coll Affiliated Hosp Dept Radiol Xuzhou 221006 Peoples R China;

    Xuzhou Med Coll Sch Med Imaging Xuzhou 221004 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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