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首页> 外文期刊>RSC Advances >Experimental study and thermodynamic modeling for purification of extracted algal lipids using an organic/aqueous two-phase system
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Experimental study and thermodynamic modeling for purification of extracted algal lipids using an organic/aqueous two-phase system

机译：用有机/水化合物水溶液纯化提取藻类脂质的实验研究及热力学建模

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The extraction and purification of lipids from the microalgae Chlorella vulgaris have been investigated. First, a mixture of hexane and ethanol was used to extract lipids from the algal biomass. Ultrasonication was employed to disrupt the cell wall and increase the extraction performance. Under these conditions, over 90% of the fatty acids in the biomass were extracted. Second, a biphasic system was formed by adding water and hexane to the extracted crude oil. In this way, fatty acids were mainly distributed in the organic phase (mostly hexane and ethanol) while most of the contaminants remained in the aqueous phase (mostly water and ethanol). Equilibrium distribution data between the phases were obtained to investigate the fatty acid lost to the aqueous phase during the purification process. The results showed that adding more water and hexane to the extraction mixture leads to a greater phase separation, as well as to higher purification of the extracted lipids. However purification efficiency did not improve very much if hexane and water were added at more than their optimum value. Two thermodynamic models (UNIQUAC and NRTL) were used in order to describe the partitioning behaviour of fatty acids in this system. The results indicated that these models can accurately estimate the fatty acid partition coefficient with average absolute deviation percentage (AAD%) of 8.69 and 9.46 for the UNIQUAC and the NRTL models, respectively. The AAD% of fatty acid recovery yield was 4.91 and 5.60 for the UNIQUAC and the NRTL models, respectively.
机译：研究了从微藻藻藻藻脂质的提取和纯化已经研究过。首先，使用己烷和乙醇的混合物从藻类生物质中提取脂质。采用超声波破坏细胞壁并提高提取性能。在这些条件下，提取了超过90％的生物质中的脂肪酸。其次，通过将水和己烷加入萃取的原油来形成双相系统。以这种方式，脂肪酸主要分布在有机相（主要是己烷和乙醇）中，而大多数污染物残留在水相（主要是水和乙醇）中。获得各相之间的平衡分布数据以研究纯化过程中损失到水相的脂肪酸。结果表明，向萃取混合物中加入更多的水和己烷导致更大的相分离，以及提取的脂质的纯化较高。然而，如果在比其最佳值的大于其最佳值的己烷和水，则净化效率并未改善。使用两个热力学模型（UNIQUAC和NRTL）以描述该系统中脂肪酸的分区行为。结果表明，这些模型可以分别准确地估计脂肪酸分区系数，分别为uniquac和NRTL模型的平均绝对偏差百分比（AAD％）为8.69和9.46。 AAD％的脂肪酸​​恢复产率为唯一的脂肪酸恢复产率为uniquac和NRTL模型分别为4.91和5.60。

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