• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection
【24h】

Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection

机译：用于超敏感人体proCalcitonin检测的多壳结构荧光复合纳米粒子的制备

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper, we reported the preparation of carboxyl functionalized quantum dots (QDs)-embedded silica nanoparticles by combining layer-by-layer (LbL) self-assembly technique and a multi-layer protection method. Preformed SiO2 nanoparticles synthesized via the Stober method were first alternately deposited with positively charged polyelectrolyte (poly(diallyl dimethylammonium chloride), PDDA) and negatively charged CdSe/ZnS QDs by LBL method and further coated by a silica shell. Then, the functionalization of SiO2@PDDA@QDs@SiO2 nanoparticles with hydrophobic hydrocarbons and biocompatible oligomers impart optical imaging properties and good colloidal stability in aqueous solutions at a wide range of pH, PBS buffer, and under thermal treatment. In addition, these newly developed multi-shell structured nanoparticles coupled with specific antibodies were successfully applied in detecting procalcitonin (PCT) antigens using fluorescent lateral flow immunoassay (LFIA). The naked-eye detection limit of fluorescence nanoparticles could achieve 0.1 ng mL(-1) of PCT antigens, which was about two hundred times higher than that of colloidal gold-based method.
机译：在本文中，通过组合逐层（LBL）自组装技术和多层保护方法，向羧基官能化量子点（QDS）-embedded二氧化硅纳米粒子的制备。首先将通过STOPE方法合成的预制的SiO2纳米颗粒用带正电的聚电解质（聚（二烯丙基二甲基氯化铵），PDDA）交替地沉积，并通过LBL方法带来带负电荷/ ZnS QDS，并通过二氧化硅壳进一步涂覆。然后，具有疏水性烃和生物相容性低聚物的SiO 2 @ PDDA QDS的官能化和生物相容性低聚物在宽范围的pH，PBS缓冲液和热处理下赋予水溶液中的光学成像性能和良好的胶体稳定性。此外，这些新开发的多壳结构化纳米颗粒与特异性抗体偶联用于使用荧光横向流动免疫测定（LFIa）检测ProCalcitonin（PCT）抗原。荧光纳米颗粒的裸眼检测极限可以实现0.1ng ml（-1）的PCT抗原，其比胶体金基方法高约两百倍。

著录项

  • 来源
    《RSC Advances》 |2015年第8期|共8页
  • 作者

    Zhao Yue; Zhou Changhua; Wu Ruili; Li Lin; Shen Huaibin; Li Lin Song;

  • 作者单位

    Henan Univ Minist Educ Key Lab Special Funct Mat Kaifeng 475004 Peoples R China;

    Henan Univ Minist Educ Key Lab Special Funct Mat Kaifeng 475004 Peoples R China;

    Henan Univ Minist Educ Key Lab Special Funct Mat Kaifeng 475004 Peoples R China;

    Autobio Diagnost Co Ltd Zhengzhou 450016 Peoples R China;

    Henan Univ Minist Educ Key Lab Special Funct Mat Kaifeng 475004 Peoples R China;

    Henan Univ Minist Educ Key Lab Special Funct Mat Kaifeng 475004 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号