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首页> 外文期刊>RSC Advances >Isolation of Bacillus amyloliquefaciens JK6 and identification of its lipopeptides surfactin for suppressing tomato bacterial wilt
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Isolation of Bacillus amyloliquefaciens JK6 and identification of its lipopeptides surfactin for suppressing tomato bacterial wilt

机译：分离芽孢杆菌淀粉胺，JK6及其脂肪肽抑制番茄细菌枯萎物的鉴定

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摘要

A rhizobacteria strain, B. amyloliquefaciens JK6, isolated from the rhizosphere soil of healthy tomato plants, significantly inhibited Ralstonia solanacearum (RS). The biocontrol efficacies (BCEs) of JK6 suppressing tomato bacterial wilt caused by RS were up to 58.6% and 52.9% in two greenhouse experiments. To evaluate the mechanisms of the antagonist, we found that JK6 could produce siderophores, protease, biofilm, cellulose, indole acetic acid (IAA) and ammonium (NH3). In addition, PCR was used to identify antimicrobial genes in JK6. Amplification products of the expected sizes were identified as srfAB, involved in surfactin synthesis; ituA, ituB, ituC and ituD, involved in iturin synthesis; fenD, involved in fengycin synthesis; and yndJ, involved in the biosynthesis of the Yndj protein. The concentration of the lipopeptides surfactin isolated from JK6 culture was 64.24 mg L-1 identified by liquid chromatography coupled with mass spectroscopy. Furthermore, the srfAB, fenD and yndJ DNA content (copy number per 100 ng of total soil DNA) in the JK6-treated soil was significantly higher than that of the controls, as determined through real-time PCR analysis. In summary, we estimated that the production of lipopeptides surfactin as well as the higher srfAB, fenD and yndJ DNA copy numbers detected in the rhizosphere soil may play major roles in the biocontrol mechanisms with which JK6 protects plants from pathogen attacking.
机译：从健康番茄植物的根际土壤中分离出的氨基杆菌菌株B.淀粉氨酸淀粉菌菌株，显着抑制了罗斯顿菌（RS）。 JK6的Biocontrol效率（BCE）抑制番茄细菌枯萎病引起的rs率均高达两个温室实验中的58.6％和52.9％。为了评估拮抗剂的机制，我们发现JK6可以产生施工团，蛋白酶，生物膜，纤维素，吲哚乙酸（IAA）和铵（NH3）。此外，PCR用于鉴定JK6中的抗微生物基因。预期尺寸的扩增产物被鉴定为SRFAB，参与Surfactin合成; ITUA，ITUB，ITUC和ITUD，参与ITurin合成; Fengycin合成伴有围绕;和YNDJ参与了YNDJ蛋白的生物合成。由JK6培养物中分离的脂肪肽表面活性素的浓度为64.24mg L-1，液相色谱法与质谱偶联。此外，通过实时PCR分析确定，JK6处理土壤中的SRFAB，Fend和每100 ng总土壤DNA的拷贝数量）显着高于对照物。总之，我们估计在根际土壤中检测到脂肪肽表面活性素以及较高的SRFAB，Fend和YNDJ DNA拷贝数可能在BioControl机制中发挥主要作用，其中JK6保护植物免受病原体攻击。

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  • 来源
    《RSC Advances》 |2015年第100期|共8页
  • 作者

    Xiong Hanqin; Li Yongtao; Cai Yanfei; Cao Yu; Wang Yan;

  • 作者单位

    South China Agr Univ Coll Nat Resources &

    Environm Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Nat Resources &

    Environm Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Nat Resources &

    Environm Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Nat Resources &

    Environm Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Nat Resources &

    Environm Guangzhou 510642 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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