• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >An amperometric immunosensor for detection of Streptococcus suis serotype 2 using a nickel-gold nanocomposite as a tracer matrix
【24h】

An amperometric immunosensor for detection of Streptococcus suis serotype 2 using a nickel-gold nanocomposite as a tracer matrix

机译：用于检测使用镍 - 金纳米复合物作为示踪基质的镍骨血管型2的静脉瘤免疫传感器

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A simple electrochemical immunosensor for determination of Streptococcus suis serotype 2 (SS2) is presented. Nickel hexacyanoferrate nanoparticles (NiHCFNPs) and gold nanocages (AuNCs) were combined together through complexation between the cyanide (CN-) and gold ions, and acted as a carrier for immobilization of the detection antibody (Ab2). The capture antibody (Ab1) was combined by a protein A (PA) and gold nanoparticle (AuNP) modified electrode. In a sandwich-type immunoassay mode, ascorbic acid (AA) was added to the base solution and its oxidation was catalyzed by the AuNCs-NiHCFNPs complex to greatly amplify the response signal. The resulting immunosensor exhibited excellent selectivity and sensitivity, with a linear concentration range of 0.0005-80 ng mL(-1) and a detection limit of 0.15 pg mL(-1).
机译：提出了一种用于测定链球菌血清型2（SS2）的简单电化学免疫传感器。 镍己酰甲醛甲醛纳米颗粒（NiHCFNP）和金纳米胺（Aunc）通过氰化物（CN-）和金离子之间的络合组合在一起，并用作固定检测抗体（AB2）的载体。 捕获抗体（AB1）由蛋白A（Pa）和金纳米颗粒（AUNP）改性电极组合。 在夹层型免疫测定模式中，将抗坏血酸（AA）加入到碱溶液中，并且其氧化由Auncs-NiHCFNPS复合物催化，大大放大响应信号。 所得的免疫传感器表现出优异的选择性和灵敏度，线性浓度范围为0.0005-80ng ml（-1）和0.15pg ml（-1）的检测限。

著录项

  • 来源
    《RSC Advances》 |2015年第97期|共6页
  • 作者

    Zhu Qiang; Chai Yaqin; Zhuo Ying; Yuan Ruo;

  • 作者单位

    Southwest Univ Coll Chem &

    Chem Engn Educ Minist Key Lab Luminescence &

    Real Time Anal Chongqing 400715 Peoples R China;

    Southwest Univ Coll Chem &

    Chem Engn Educ Minist Key Lab Luminescence &

    Real Time Anal Chongqing 400715 Peoples R China;

    Southwest Univ Coll Chem &

    Chem Engn Educ Minist Key Lab Luminescence &

    Real Time Anal Chongqing 400715 Peoples R China;

    Southwest Univ Coll Chem &

    Chem Engn Educ Minist Key Lab Luminescence &

    Real Time Anal Chongqing 400715 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号