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首页> 外文期刊>RSC Advances >Electrochemical detection of cholesterol based on competitive host-guest recognition using a beta-cyclodextrin/poly(N-acetylaniline)/graphene-modified electrode
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Electrochemical detection of cholesterol based on competitive host-guest recognition using a beta-cyclodextrin/poly(N-acetylaniline)/graphene-modified electrode

机译：使用β-环糊精/聚（N-乙酰苯胺）/石墨烯改性电极基于竞争性宿主 - 访客识别的胆固醇的电化学检测

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摘要

A sensitive and selective electrochemical approach for cholesterol sensing based on a competitive host-guest recognition between beta-cyclodextrin (beta-CD) and a signal probe (methylene blue)/target molecule (cholesterol) using a beta-CD/poly(N-acetylaniline)/graphene (beta-CD/PNAANI/Gra)-modified electrode was developed. Due to the host-guest interaction, MB molecules can enter into the hydrophobic inner cavity of beta-CD, and the beta-CD/PNAANI/Gra modified glassy carbon electrode displays a remarkable anodic peak. In the presence of cholesterol, a competitive interaction to beta-CD occurs and the MB molecules are displaced by cholesterol. This results in a decreased oxidation peak current of MB as MB is a well known redox probe and hence can be easily detected using the differential pulse voltammetery technique. A linear response range of 1.00 to 50.00 mu M for cholesterol with a low detection limit of 0.50 mu M (S/N = 3) was obtained by using the indirect method. The proposed method could be successfully utilized to detect cholesterol in serum samples, and may be expanded to analysis of other non-electroactive species. Besides, the host-guest interaction between cholesterol and beta-CD was studied by molecular modeling calculations, which revealed that the complexation could reduce the energy of the system and the complex of 2 : 1 host-guest stoichiometry had the lowest DE value of -10.45 kcal mol(-1). The molecular docking studies suggested that hydrogen bonding, electrostatic interactions, and hydrophobic interactions should be the major driving forces for the formation of the inclusion complex.
机译：对于胆固醇甲灵敏度和选择性电化学靠近感测基于β-环糊精（β-CD）和信号探针（亚甲基蓝）/使用的β-CD /聚靶分子（胆固醇）之间的竞争性主客体识别（N-乙酰苯胺）/石墨烯（β-CD / PNAANI / GRA）改性的电极的开发。由于主 - 客体相互作用，MB分子可进入的β-CD的疏水内部空腔，且β-CD / PNAANI / GRA修饰玻碳电极显示显着的氧化峰。在胆固醇的存在下，于β-CD具有竞争力的相互作用发生，并且MB分子通过胆固醇移位。作为MB是一个众所周知的氧化还原探针，因此可以很容易地使用差分脉冲voltammetery技术检测这导致降低的MB的氧化峰电流。的1.00至50.00微米的线性响应范围M为0.50微米（S / N = 3）的低检测限胆固醇通过使用间接方法获得的。所提出的方法可以成功地用于检测血清样品中的胆固醇，并可以扩展到其他非电活性物质的分析。此外，胆固醇和β-CD之间的主客体相互作用研究通过分子模拟计算，这表明，络合可以减少系统的能量和复杂的2：1主客体化学计量有最低DE值 - 10.45千卡摩尔（-1）。分子对接研究表明，氢键，静电相互作用和疏水相互作用应的主要驱动力为包合物的形成。

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  • 来源
    《RSC Advances》 |2015年第79期|共10页
  • 作者

    Yang Long; Zhao Hui; Fan Shuangmei; Zhao Genfu; Ran Xin; Li Can-Peng;

  • 作者单位

    Yunnan Univ Sch Chem Sci &

    Technol Kunming 650091 Peoples R China;

    Yunnan Univ Lab Conservat &

    Utilizat Bioresource Kunming 650091 Peoples R China;

    Yunnan Univ Sch Chem Sci &

    Technol Kunming 650091 Peoples R China;

    Yunnan Univ Sch Chem Sci &

    Technol Kunming 650091 Peoples R China;

    Yunnan Univ Sch Chem Sci &

    Technol Kunming 650091 Peoples R China;

    Yunnan Univ Sch Chem Sci &

    Technol Kunming 650091 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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