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首页> 外文期刊>RSC Advances >Investigation of antifouling universality of polyvinyl formal (PVF) membranes utilizing atomic force microscope (AFM) force curves
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Investigation of antifouling universality of polyvinyl formal (PVF) membranes utilizing atomic force microscope (AFM) force curves

机译：利用原子力显微镜（AFM）力曲线的聚乙烯族（PVF）膜防污均升级性研究

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摘要

As a nano-Newton level force sensor, an AFM probe with a protein-modified tip could be used to investigate the antifouling properties of different ultrafiltration membranes. In this paper, the spring constants of the cantilevers of AFM probes were calibrated with a SI-traceable measurement system consisting of a precision balance, an optical lever and nano-positioning stages. The calibration system for the measured AFM cantilevers has less than 3% uncertainty, highly narrowing the large range given by the distributers. Thus, the adhesion forces between the AFM probes with different immobilized proteins and various ultrafiltration membranes could be compared with each other using force curves. The antifouling universality of a specific membrane (polyvinyl formal, PVF) towards multiple proteins (bovine serum albumin, lysozyme, catalase and fibrinogen) could be studied comprehensively. The results reveal that the antifouling property of membranes becomes better through blending F127 with PVF for all four foulants, so the antifouling universality of the membrane was improved effectively. The membrane with 60% F127 addition had the best antifouling properties. Comparison experiments of protein absorption and water contact angle have also been done. As the F127 additive varied from 0% to 60%, the amount of protein adsorption on the surface of the membranes decreased significantly and the water contact angle decreased from 58.6 degrees to 47.1 degrees, which indicated that the blend membranes had better antifouling universality. The experimental results agreed with AFM force curve experiments very well.
机译：作为纳米级牛顿力传感器，用蛋白质修饰的末端的原子力显微镜探针可用于研究不同超滤膜的防污性能。在本文中，AFM探针的悬臂的弹簧常数用由精密天平，光杆和纳米级定位的SI-可追溯的测量系统校准。对于所测量的AFM悬臂的校准系统具有小于3％的不确定性，高度缩小由发布者提供的大范围。因此，具有不同的固定化的蛋白质和多种超滤膜的AFM探针之间的粘合力可以相互使用力曲线进行比较。一个特定膜的防污普遍性（聚乙烯醇缩甲醛，PVF）向多种蛋白质（牛血清白蛋白，溶菌酶，过氧化氢酶和纤维蛋白原），可以全面的研究。结果表明，膜的耐污染性通过与PVF混合F127为所有四个污垢变得更好，所以膜的防污普遍性得到有效改善。有60％的F127除了薄膜具有最好的防污性能。蛋白质的吸收和水的接触角的比较试验也已经完成。作为F127添加剂从0％变化至60％，蛋白质吸附膜的表面上的量降低显著和水接触角从58.6度下降到47.1度，这表明该共混物膜最好防污普遍性。实验结果与AFM力曲线实验同意很好。

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  • 来源
    《RSC Advances》 |2015年第46期|共8页
  • 作者

    Liu Yunqiang; Xu Linyan; Song Yunpeng; Fu Xing; Zou Jing; Hu Xiaotang; Jiang Zhongyi; Zhao Xueting;

  • 作者单位

    Tianjin Univ State Key Lab Precis Measuring Technol &

    Instrume Tianjin 300072 Peoples R China;

    Tianjin Univ State Key Lab Precis Measuring Technol &

    Instrume Tianjin 300072 Peoples R China;

    Tianjin Univ State Key Lab Precis Measuring Technol &

    Instrume Tianjin 300072 Peoples R China;

    Tianjin Univ State Key Lab Precis Measuring Technol &

    Instrume Tianjin 300072 Peoples R China;

    Tianjin Univ State Key Lab Precis Measuring Technol &

    Instrume Tianjin 300072 Peoples R China;

    Tianjin Univ State Key Lab Precis Measuring Technol &

    Instrume Tianjin 300072 Peoples R China;

    Tianjin Univ Coll Chem Engn &

    Technol Minist Educ Key Lab Green Chem Technol Tianjin 300072 Peoples R China;

    Tianjin Univ Coll Chem Engn &

    Technol Minist Educ Key Lab Green Chem Technol Tianjin 300072 Peoples R China;

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