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首页> 外文期刊>RSC Advances >Detection of Staphylococcus aureus using acridine orange-doped silica nanoparticles as a fluorescent label
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Detection of Staphylococcus aureus using acridine orange-doped silica nanoparticles as a fluorescent label

机译：用吖啶橙掺杂二氧化硅纳米粒子作为荧光标记检测金黄色葡萄球菌

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摘要

In this paper, a new type of core-shell fluorescent silica nanoparticles (NPs) carrying amino groups on the surface was designed and prepared by an inverse-microemulsion method using acridine orange (AO) as the dopant fluorophore. These NPs are monodisperse and possess a uniform diameter of similar to 80 nm. The introduction of amino groups on the nanoparticles surfaces was proven by IR spectroscopy. Fluorescence studies indicate that the photostability of the NPs was improved compared to that of AO. A highly sensitive, simple and rapid counting approach for Staphylococcus aureus (S. aureus) was established by using the NPs as a fluorescent label. Fluorescence intensity and the total number of S. aureus are linearly correlated within the range of 10(3) to 10(7) cfu mL(-1), and the limit of detection is 500 cfu mL(-1). The recovery rate for a spiked sample ranged from 96.5% to 102.7%, and the RSD was lower than 7% (for n = 7). We presume that this method has a large potential for further applications to rapid analysis of pathogens.
机译：本文通过吖啶橙（AO）作为掺杂剂荧光团，设计并制备了一种新型核 - 壳荧光二氧化硅纳米粒子（NPS），其载有吖啶橙（AO）作为掺杂剂荧光团。这些NPS是单分散的并且具有与80nm相似的均匀直径。通过IR光谱证实了纳米颗粒表面上的氨基的引入。荧光研究表明，与AO相比，NP的光稳定性得到改善。通过使用NPS作为荧光标记来建立高度敏感，简单，快速计数的金黄色葡萄球菌（金黄色葡萄球菌）。荧光强度和S.UUREUS的总数在10（3）至10（7）CFU mL（-1）的范围内是线性相关的，并且检测限为500 cfu ml（-1）。尖刺样品的回收率范围为96.5％至102.7％，RSD低于7％（对于n = 7）。我们假设该方法具有巨大的应用，以进一步应用于快速分析病原体。

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  • 来源
    《RSC Advances》 |2015年第67期|共7页
  • 作者

    Jiang Yan; Xu Yi; Wang Renjie; Zhao Bin; Zhang XiaoDan; Zhao Huazhou;

  • 作者单位

    CQU Chem &

    Chem Engn Chongqing 400044 Peoples R China;

    CQU Chem &

    Chem Engn Chongqing 400044 Peoples R China;

    CQU Chem &

    Chem Engn Chongqing 400044 Peoples R China;

    CQU Chem &

    Chem Engn Chongqing 400044 Peoples R China;

    CQU Chem &

    Chem Engn Chongqing 400044 Peoples R China;

    CQU Chem &

    Chem Engn Chongqing 400044 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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