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De novo synthesis of novel bacteriogenic nanocell particles and its cancer cell compatibility evaluation

机译：新型菌丝纳米细胞颗粒的德无外合成及其癌细胞兼容性评价

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摘要

This study demonstrates the effective synthesis of nanocell particles of bacterial origin using an eco-friendly ultrasonic approach. The synthesized particles were separated by sequential centrifugation. The probe sonication technique with 5 min sonication time at 20 kHz frequency successfully yielded regular spherical shaped organic nanoparticles directly from bacterial biomass in the 33-42 nm size range. The holding capacity of the particles was verified by synthesizing the particles in the presence of acridine orange (AO) and further characterized using FTIR and fluorescence spectroscopy. The internalization ability of all the three different sized particles separated after sonication by differential centrifugation was elaborately investigated on HeLa cells using confocal laser scanning microscopy. Out of the three different particles tested, the nanosized particles exhibited maximum internalization capacity. The toxicity/biocompatibility on HeLa cancer cells was studied using MTT assay and the results substantiated that all the particles were highly biocompatible. The results confirm that the indigenously synthesized nanocell particles can be used as efficient drug carriers for drug delivery for cancer cells.
机译：本研究通过生态友好的超声方法表明了有效合成了细菌来源的纳米细胞颗粒。通过顺序离心分离合成的颗粒。具有5分钟的超声处理时间为20kHz频率的探针超声处理技术直接从33-42nm尺寸范围内直接从细菌生物量产生规则的球形有机纳米颗粒。通过在吖啶橙（AO）存在下合成颗粒并进一步使用FTIR和荧光光谱来验证颗粒的保持能力。通过差动离心在超声分离后分离的所有三种不同尺寸颗粒的内化能力在HeLa细胞上进行了鉴别的研究，使用共聚焦激光扫描显微镜检查。在测试的三种不同的颗粒中，纳米化颗粒具有最大的内化容量。使用MTT测定研究了HeLa癌细胞上的毒性/生物相容性，结果证实了所有颗粒是高度生物相容性的。结果证实，本土合成的纳米细胞颗粒可用作癌细胞的药物递送的有效药物载体。

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  • 来源
    《RSC Advances》 |2015年第97期|共8页
  • 作者

    Manikandan Muthu; Ramachandran D.; Chun Sechul;

  • 作者单位

    Konkuk Univ Dept Bioresource &

    Food Sci Seoul 143701 South Korea;

    Sathyabama Univ Ctr Nanosci &

    Nanotechnol Madras 600119 Tamil Nadu India;

    Konkuk Univ Dept Bioresource &

    Food Sci Seoul 143701 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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