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Microbial production of phenol via salicylate decarboxylation

机译：通过水杨酸盐脱羧苯酚的微生物生产

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摘要

Phenol, as an important bulk chemical, is the synthetic precursor to polycarbonates, epoxide resins and phenolic resins. Currently, phenol production relies on chemical processes using benzene as the starting material, which are environmentally incompatible and nonrenewable. Here we designed a novel phenol biosynthetic pathway and achieved phenol production from renewable carbon sources in Escherichia coli. A decarboxylase from Klebsiella pneumoniae was over-expressed and purified. In vitro assay was conducted to determine the kinetic parameters toward salicylate. In the bioconversion study, 1154.5 +/- 12.0 mg L-1 of phenol was produced from supplemented salicylate. Connecting salicylate decarboxylation with its biosynthesis led to microbial phenol production. Phenol production was improved by enhancing upstream shikimate pathway and modulating gene expression levels. E. coli strain QH4 was a better strain than BW25113 for phenol production. In shake flask experiment the best strain produced 405.6 +/- 13.7 mg L-1 of phenol with only trace amount of salicylate left in the culture. Optimization of cultivation conditions further increase phenol titer by 16.4% to 472.1 +/- 19.8 mg L-1. This study provides a promising alternative for sustainable production of phenol.
机译：作为一种重要的散装化学品，苯酚是聚碳酸酯，环氧化物树脂和酚醛树脂的合成前体。目前，苯酚产量依赖于使用苯作为原料的化学方法，这些方法是环境不相容和不可再生的。在这里，我们设计了一种新型酚类生物合成途径，并从大肠杆菌中获得了可再生碳源的酚类生产。来自Klebsiella肺炎的脱羧酶过度表达并纯化。进行体外测定以确定朝向水杨酸盐的动力学参数。在生物转化研究中，由补充的水杨酸盐制备1154.5 +/- 12.0mg L-1苯酚。将水杨酸盐脱羧与其生物合成导致微生物酚类产生。通过增强上游Shikimate途径和调节基因表达水平，改善了酚类产量。大肠杆菌菌株QH4比BW25113更好的苯酚生产。在摇瓶实验中，最好的应变产生405.6 +/- 13.7mg L-1的苯酚，只有含有痕量的水杨酸盐留在培养物中。优化培养条件进一步将苯酚滴度增加16.4％至472.1 +/- 19.8mg L-1。本研究为可持续生产的苯酚提供了有希望的替代方案。

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  • 来源
    《RSC Advances》 |2015年第112期|共5页
  • 作者

    Ren Yanxian; Yang Sen; Yuan Qipeng; Sun Xinxiao;

  • 作者单位

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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