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首页> 外文期刊>RSC Advances >Fabrication of multi-layered DNA nanostructures using single-strand and double-crossover tile connectors
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Fabrication of multi-layered DNA nanostructures using single-strand and double-crossover tile connectors

机译：使用单轴和双交叉瓦连接器制备多层DNA纳米结构

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摘要

DNA is an excellent and extraordinarily versatile building block that can be used to construct nanoscale objects and arrays of increasing complexity, and as a result, a considerable amount of progress has been made in DNA-directed molecular self-assembly. Here, we demonstrate the sequential fabrication of three-dimensional multilayered DNA nanostructures by utilizing single-strand and double-crossover tile (DX) designs via substrate-assisted growth and multistep annealing methods. We used both layering and connector tiles to synthesize the base layer for both the single strand-based and DX tile-based designs. Layering without and with connector tiles was used to produce double-layer and multi-layer designs for single strand based designs, but only layering tiles were used for the DX tile-based design. Connector tiles provided appropriate sticky-end sets to form the designed lattice structures. Atomic force microscopy revealed that the spacing between the tiles was in good agreement with the design scheme, but the heights of the multi-layered nanostructures were found to be slightly lower than expected due to suppression by the substrate. This kind of step-wise multi-layer assembly may produce a variety of spacings to incorporate different guest molecules or aid the attachment of various types of biomolecules and nanomaterials in parallel arrays along the layers.
机译：DNA是一种优异且非常通用的构建块，可用于构建纳米级目的和增加复杂性的阵列，结果，在DNA定向的分子自组装中已经进行了大量的进展。这里，我们通过利用单链和双交叉瓦（DX）设计通过基材辅助生长和多步骤退火方法来证明三维多层DNA纳米结构的连续制造。我们使用分层和连接器块来合成基于单链和基于DX平铺的设计的基础层。用于没有连接器瓦片的分层用于为单链的基于股线设计进行双层和多层设计，但仅用于分层砖用于DX Tile的设计。连接器瓷砖提供了适当的粘性末端组，以形成设计的晶格结构。原子力显微镜显示，瓷砖之间的间距与设计方案吻合良好，但由于基材抑制，发现多层纳米结构的高度略低于预期。这种逐步的多层组件可以产生各种间隔，以结合不同的客体分子，或者有助于沿着层的平行阵列附着各种类型的生物分子和纳米材料。

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