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Enhancement of thermal and pH stability of an alkaline metalloprotease by nano-hydroxyapatite and its potential applications

机译：纳米羟基磷灰石及其潜在应用增强碱金属蛋白酶的热和pH稳定性

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摘要

A purified calcium metalloprotease from Bacillus subtilis AKP(N) supplemented with hydroxyapatite nanoparticles [protease (+NP)] showed remarkable increase in thermostability (at 90 ~C) over a broad alkaline pH range as compared to the non-supplemented enzyme. Thermodynamic analysis of the protease (+NP) showed decreases in Km and activation energy (Ea) with increased Vmax and deactivation energy (Ed) at both 40 ~C and 90 ~C. The protease (+NP) exhibited compatibility with various laboratory and commercial detergents (1%) at both 40 ~C and 90 ~C. The protease (+NP) was utilized to efficiently degrade feather to produce amino acids, removal of blood stains from cloth and tenderization of raw meat within a short time.
机译：与羟基磷灰石纳米颗粒的芽孢杆菌Akp（n）的纯化的钙金属蛋白酶[蛋白酶（+ np）]显示出与非补充酶相比在宽碱性pH范围内的热稳定性（在90℃）上显着增加。 蛋白酶（+ NP）的热力学分析显示km和活化能（EA）的降低，在40〜C和90℃下增加VMAX和失活能量（ED）。 蛋白酶（+ NP）在40〜C和90℃下表现出与各种实验室和商业洗涤剂（1％）的相容性。 蛋白酶（+ NP）用于有效降解羽毛以产生氨基酸，从短时间内从布料中除去血液污渍并使生肉的嫩化。

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  • 来源
    《RSC Advances》 |2015年第109期|共17页
  • 作者

    Arka Mukhopadhyay; Krishanu Chakrabarti;

  • 作者单位

    Department of Biochemistry and Microbiology Rhodes University Grahamstown-6140 South Africa;

    Department of Biochemistry University College of Science Calcutta University 35 Ballygunge Circular Road West Bengal Kolkata 700 019 India.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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