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首页> 外文期刊>RSC Advances >Total phenolic content, antioxidant capacity and phytochemical profiling of grape and pomegranate wines
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Total phenolic content, antioxidant capacity and phytochemical profiling of grape and pomegranate wines

机译：葡萄树和石榴葡萄酒的总酚类含量，抗氧化能力和植物化学仿形

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The object of this study was to determine the phenolic profile, the total phenolic content and antioxidant capacity of pomegranate wine and compare to multi-varietal red wine using different spectrophotometric and spectrometric techniques. Total phenolic content was determined by the Folin-Ciocalteu assay. The antioxidant capacity was measured by the DPPH and the ABTS radical scavenging assays. The radicalscavenging capacity was higher for pomegranate wine (statistically significant difference was observed for the DPPH assay) in agreement with its higher total phenolic content (383.19 +/- 18.22 and 296.57 +/- 25.23 mg gallic acid equivalents/100 mL for pomegranate and grape wine respectively). Customized HPLC-PDA-ESI-MSn and GC-MS methods were applied for the identification and chemical characterization of the phenolic compounds for both wines. Identification using LC-MS was based on their lambda(max) (nm) and the characteristic fragments which derived from the sequential fragmentation in MS while GC-MS was based on commercial libraries and mass spectra of authentic standards. Eighty one different phenolic compounds were characterised by LC-MS and one hundred eight compounds by GCMS after different chemical hydrolysis regimes. The study signifies the prior treatment with alkaline hydrolysis which had a considerable effect on the detection of phenolic compounds. The results showed that the combination of LC-MS and GC-MS methods allowed the detection of different compounds while results from both techniques are complementary and may confirm each other. Phytochemicals with proven biological activities including antimicrobial, antiviral and chemoprotective, have been identified mainly in pomegranate wines. Furthermore, a significant diversity between pomegranate and grape wines was observed, in terms of their phenolic content and antioxidant profiles indicating thenutritive and health-promoting effects of pomegranate wine.
机译：本研究的目的是确定酚醛化葡萄酒的酚醛概况，总酚类含量和抗氧化能力，并使用不同的分光光度和光谱技术与多品种红葡萄酒进行比较。通过Folin-Ciocalteu测定法测定总酚醛含量。通过DPPH和ABTS自由基清除测定测量抗氧化能力。对于石榴葡萄酒（DPPH测定观察到统计学上差异）的激热肉类活力与其较高的总酚醛含量（383.19 +/- 18.22和296.57 +/- 25.23 mg加仑的石榴，用于石榴和葡萄的统计学意义葡萄酒分别）。施用定制的HPLC-PDA-ESI-MSN和GC-MS方法，用于鉴定和化学表征两种葡萄酒的酚类化合物。使用LC-MS的鉴定基于它们的λ（MAX）（nm）和衍生自MS中顺序碎片的特征片段，而GC-MS基于商业文库和正宗标准的质谱。通过LC-MS和通过GCMS在不同化学水解制度之后的GCMS表征八十个不同的酚类化合物。该研究表明了碱性水解的先前治疗，对酚类化合物的检测具有相当大的影响。结果表明，LC-MS和GC-MS方法的组合允许检测不同化合物，同时来自两种技术的结果是互补的，并且可以互相确认。具有经过验证的生物活性的植物化学品，包括抗微生物，抗病毒和化学防护，主要是在石榴葡萄酒中鉴定。此外，就其酚醛含量和抗氧化谱而言，观察到石榴和葡萄葡萄葡萄葡萄葡萄葡萄葡萄葡萄葡萄葡萄酒之间的显着多样性。表明石榴葡萄酒的福利和健康促进作用。

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