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首页> 外文期刊>RSC Advances >Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures
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Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures

机译：将临床等级磁性纳米颗粒与磁场部署到磁性标签神经干细胞粘附与悬浮培养物中

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摘要

Neural stem cells (NSCs) have a high therapeutic potential for patients with neurological disease/injury given their neuroregenerative and immunomodulatory capabilities. In recent years, magnetic nanoparticles (MNPs) have been used as contrast agents in translational studies, to track transplanted NSCs using non-invasive magnetic resonance imaging (MRI). However, NSC uptake of MNPs is inherently low in the absence of chemical/biological uptake enhancing strategies such as cell targeting peptides and transfection agents - approaches which may be cytotoxic and alter cellular physiology. By contrast, physical delivery strategies relying on magnetic assistive methods can safely enhance MNP uptake into multiple neural cell types. The utility of this approach has been demonstrated for gene delivery grade particles but their application in enhancing 'magnetolabelling' with clinical grade contrast agents has never been evaluated. Here, we show that applied oscillating magnetic fields can safely enhance the uptake of a clinical grade MNP (Lumirem/Ferumoxsil) into NSCs propagated as neurospheres (suspension cultures, the preferred format for transplantation) but offer limited benefit for monolayer (adherent) cultures. This physical delivery method therefore has potential to facilitate cell labelling for clinical therapies.
机译：神经干细胞（NSCs）对神经疾病和免疫调节能力的患者具有高治疗潜力。近年来，磁性纳米颗粒（MNP）已被用作平移研究中的造影剂，用于使用非侵入性磁共振成像（MRI）进行移植的NSC。然而，在没有化学/生物摄取增强诸如细胞靶向肽和转染剂的诸如细胞毒性和改变细胞生理学的方法的情况下，MNP的NSC吸收本质上是低的。相比之下，依赖于磁辅助方法的物理交付策略可以安全地增强MNP吸收成多种神经细胞类型。这种方法的效用已经证明了基因递送级颗粒，但它们在增强具有临床级造影剂的“磁性标签”中的应用从未得到评估。在这里，我们表明，施加的振荡磁场可以安全地增强临床级MNP（Lumirem / FerumoxsIL）进入NSCs作为神经球（悬浮培养物，移植的优选形式），但为单层（粘附剂）培养物提供有限的益处。因此，该物理递送方法具有促进临床疗法的细胞标记的可能性。

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  • 来源
    《RSC Advances》 |2015年第54期|共8页
  • 作者

    Weinberg D.; Adams C. F.; Chari D. M.;

  • 作者单位

    Keele Univ Inst Sci &

    Technol Med Cellular &

    Neural Engn Grp Keele ST5 5BG Staffs England;

    Keele Univ Inst Sci &

    Technol Med Cellular &

    Neural Engn Grp Keele ST5 5BG Staffs England;

    Keele Univ Inst Sci &

    Technol Med Cellular &

    Neural Engn Grp Keele ST5 5BG Staffs England;

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  • 正文语种 eng
  • 中图分类 化学;
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