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首页> 外文期刊>RSC Advances >One-step cross-linked injectable hydrogels with tunable properties for space-filling scaffolds in tissue engineering
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One-step cross-linked injectable hydrogels with tunable properties for space-filling scaffolds in tissue engineering

机译：组织工程中具有可调谐性能的一步交联注射水凝胶，用于组织工程中的空间填充支架

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摘要

Developments in tissue engineering have led to the fabrication and refinement of a wide variety of injectable space-filling agents, as they can be injected to the tissue defect sites in a minimally invasive manner. Hydrogels are promising since the native tissue composed of cells and extracellular matrix can be well simulated by hydrogels encapsulating therapeutic cells and drugs. In this study, aldehyde dextran (ald-dex) and amino gelatin (ami-gel) were prepared through reaction with sodium periodate and ethane diamine to fabricate in situ gelable ald-dex/ami-gel hydrogel. The gelation process was monitored rheologically and the microstructure was revealed under scanning electron microscopy (SEM). The tunable porous structures were obtained through simply altering the ratio of aldehyde dextran and amino gelatin. Gelation time and swelling ratio of the ald-dex/ami-gel hydrogels were shown to be related to the crosslinking density of the hydrogels, which can be modulated through simply changing the proportion of ald-dex/ami-gel. The degradation rate could also be modulated by altering the content of aldehyde dextran. Then biocompatibility evaluation was performed on both two-dimensional (2D) and three-dimensional (3D) environments by using mouse pre-osteoblast cells (MC3T3-E1). According to the results, beneficial cell responses such as the attachment, spreading and proliferation of cells were exhibited with the increase of the gelatin content. Furthermore, MC3T3-E1 cells encapsulated inside the hydrogel assumed a round shape initially and gradually adapted to the microenvironment to spread out. Therefore, the ability to support cell adhesion and spreading on the surface and long-term viability of encapsulated cells enabled the tunable ald-dex/ami-gel hydrogel to be an effective space-filling scaffold for tissue engineering.
机译：组织工程的发展导致了各种可注射空间填充剂的制造和改进，因为它们可以以微创的方式注射到组织缺陷位点。水凝胶是有前途的，因为由细胞和细胞外基质组成的天然组织可以通过包封治疗细胞和药物的水凝胶进行良好的模拟。在该研究中，通过与钠碘酸钠和乙烷二胺反应制备醛葡聚糖（ALD-DEX）和氨基明胶（AMI-凝胶），以制造原位可胶型ALD-DEX / AMI-凝胶水凝胶。使凝胶化过程是流变学监测，并且在扫描电子显微镜（SEM）下显露微观结构。通过简单地改变醛葡聚糖和氨基明胶的比例获得可调谐多孔结构。 AlD-DEX / AMI-凝胶水凝胶的凝胶时间和溶胀比显示与水凝胶的交联密度有关，其可以通过简单地改变ALD-DEX / AMI-凝胶的比例来调节。还可以通过改变醛葡聚糖的含量来调节降解速率。然后通过使用小鼠预成骨细胞（MC3T3-E1）对二维（2D）和三维（3D）环境进行生物相容性评价。根据结果​​，随着明胶含量的增加，表现出诸如细胞的附着，扩散和增殖的有益细胞反应。此外，封装在水凝胶内部的MC3T3-E1细胞最初假设圆形并逐渐适应于微环境以扩散。因此，将细胞粘附和扩散在包封细胞的表面和长期活力上的能力使可调谐的ALD-DEX / AMI-凝胶水凝胶成为用于组织工程的有效空间填充支架。

著录项

  • 来源
    《RSC Advances》 |2015年第51期|共11页
  • 作者

    Pan Jian-feng; Yuan Heng-feng; Guo Chang-an; Liu Jia; Geng Xiao-hua; Fei Teng; Li Shuo; Fan Wen-shuai; Mo Xiu-mei; Yan Zuo-qin;

  • 作者单位

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

    East City Hosp Yangpu Dist Dept Orthoped Shanghai 200438 Peoples R China;

    Donghua Univ State Key Lab Modificat Chem Fibers &

    Polymer Mat Coll Mat Sci &

    Engn Shanghai 201620 Peoples R China;

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

    Donghua Univ State Key Lab Modificat Chem Fibers &

    Polymer Mat Coll Mat Sci &

    Engn Shanghai 201620 Peoples R China;

    Zhongshan Hosp Dept Orthoped Shanghai 200032 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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