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首页> 外文期刊>RSC Advances >Photoluminescent graphene quantum dots for in vitro and in vivo bioimaging using long wavelength emission
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Photoluminescent graphene quantum dots for in vitro and in vivo bioimaging using long wavelength emission

机译：使用长波长发射的体外和体内生物模拟的光致发光石墨烯量子点

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摘要

Graphene quantum dots (GQDs), due to their ultrasmall size, excellent optical properties, chemical stability, biocompatibility, anti-photobleaching as well as low toxicity, have been widely used as fluorescent bioprobes. In this study, we used the top-down "nano-cutting" route to prepare fluorescent GQDs. The as-prepared GQDs possessed ca. 4 nm diameter with 0.218 nm crystal lattice constant, and they had outstanding solubility as many oxygen and nitrogen based groups were present on them. The GQDs were exploited in bioimaging in vitro and in vivo. Using rat Schwann cells as the model system, the time-dependent cellular uptake of the GQDs was tested by a fluorescence activated cell sorter (FACS) and confocal laser scanning microscope (CLSM), and it reached the saturation point after 24 h. Benefiting from the excitation-dependent PL of the GQDs, multi-color cell labeling was achieved, and the GQDs were proved to be mainly distributed in the cytoplasm and lysosomes. Furthermore, using long wavelength emission (620 nm), in vivo imaging was realized in nude mice.
机译：石墨烯量子点（GQDS），由于它们的超大尺寸，优异的光学性质，化学稳定性，生物相容性，抗光漂等以及低毒性，已被广泛用作荧光生物体。在这项研究中，我们使用了自上而下的“纳米切割”途径来制备荧光GQD。准备的GQD具有CA.具有0.218nm晶格常数的4nm直径，并且它们具有出色的溶解度，因为它们上存在许多氧气和氮基团。 GQDS在体外和体内在生物模仿中被利用。使用RAT Schwann细胞作为模型系统，通过荧光激活的细胞分选机（FACS）和共聚焦激光扫描显微镜（CLSM）测试GQD的时间依赖性蜂窝摄取，并且在24小时后达到饱和点。从GQDS的激发依赖性PL受益，实现了多色细胞标记，并证明了GQDS主要分布在细胞质和溶酶体中。此外，使用长波长发射（620nm），在裸鼠中实现体内成像。

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  • 来源
    《RSC Advances》 |2015年第49期|共5页
  • 作者

    Zhu Shoujun; Zhou Nan; Hao Zeyu; Maharjan Suraj; Zhao Xiaohuan; Song Yubin; Sun Bin; Zhang Kai; Zhang Junhu; Sun Hongchen; Lu Laijin; Yang Bai;

  • 作者单位

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Hosp 1 Dept Hand Surg Changchun 130012 Peoples R China;

    Jilin Univ Hosp 1 Dept Hand Surg Changchun 130012 Peoples R China;

    Jilin Univ Hosp 1 Dept Hand Surg Changchun 130012 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Sch Stomatol Changchun 130041 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

    Jilin Univ Sch Stomatol Changchun 130041 Peoples R China;

    Jilin Univ Hosp 1 Dept Hand Surg Changchun 130012 Peoples R China;

    Jilin Univ Coll Chem State Key Lab Supramol Struct &

    Mat Changchun 130012 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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