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Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

机译：纳米宽容性平台，用于微升压中的准确热化学研究

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摘要

A straightforward and general way for monitoring chemical reactions is via their thermal signature. Such approach requires however an experimental setup with a high thermal stability that simultaneously allows time-resolved heat detection with high sensitivity. We present a nanocalorimetric platform for accurate thermochemical studies of (bio-) chemical reactions in a miniaturized format (tens of microliter volume), characterized by a fast thermalization time to a preset temperature (<30 minutes), an excellent base temperature stability (+/- 1 mK) and a fast sensing response time (few seconds). The platform is built around a commercial thermopile-based sensor chip, on which an open-well reservoir holding the sample is directly positioned. The sample is, prior to the experiment, pipetted into the reservoir, in which small aliquots of reagents are injected subsequently and sequentially via thermalized microfluidic conducts. The design of the platform is optimized by means of numerical simulations. Via thermoelectric calibration using a resistive heater positioned either on the sensor chip or in the reservoir, we obtain a maximum power sensitivity of 2.7 V W-1 and a heat limit of detection of 70 nW. The excellent functionality of the platform is demonstrated by measuring the reaction enthalpy of 1-propanol in water and the rate constant k and enthalpy change of the oxidation reaction of glucose catalyzed by glucose oxidase, showing good agreement with literature data. Our versatile platform may be applied to many thermochemical studies, including thermodynamic analysis and kinetic reaction analysis, and its ease of use will allow implementation of many different experimental protocols.
机译：用于监测化学反应的直接和通用方式是通过其热签名。然而，这种方法需要具有高热稳定性的实验设置，同时允许具有高灵敏度的时间分辨热检测。我们提出了一种用于以小型化形式（数十微升体积）的精确热化学研究（生物）化学反应的准确热化学研究，其特征在于预设温度（<30分钟），优异的基础温度稳定性（+ / - 1 mk）和快速传感响应时间（几秒钟）。该平台围绕商用热电堆的传感器芯片构建，其中保持样品的开放井储存器直接定位。在实验之前，样品在储存到贮存器之前，其中随后通过热化的微流体传导依次注射小等分试剂。通过数值模拟优化平台的设计。通过使用电阻加热器在传感器芯片或储存器上定位的电阻加热器的热电校准，我们获得2.7V W-1的最大功率灵敏度和70nW检测的热量。通过测量水中的1-丙醇的反应焓和葡萄糖氧化酶催化的葡萄糖氧化反应的速率常数K和焓变化来证明平台的优异功能，表现出与文献数据的良好一致性。我们的多功能平台可以应用于许多热力学研究，包括热力学分析和动力学反应分析，其易用性将允许实施许多不同的实验方案。

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  • 来源
    《RSC Advances》 |2015年第118期|共10页
  • 作者

    Padovani Rima; Lehnert Thomas; Trouillon Raphael; Gijs Martin A. M.;

  • 作者单位

    Ecole Polytech Fed Lausanne Lab Microsyst CH-1015 Lausanne Switzerland;

    Ecole Polytech Fed Lausanne Lab Microsyst CH-1015 Lausanne Switzerland;

    Ecole Polytech Fed Lausanne Lab Microsyst CH-1015 Lausanne Switzerland;

    Ecole Polytech Fed Lausanne Lab Microsyst CH-1015 Lausanne Switzerland;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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