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首页> 外文期刊>RSC Advances >A fluorescence nanosensor for lipase activity: enzyme-regulated quantum dots growth in situ
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A fluorescence nanosensor for lipase activity: enzyme-regulated quantum dots growth in situ

机译：脂肪酶活性的荧光纳米传感器：酶调节量子点原位生长

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摘要

Lipase is a neglected enzyme in the field of quantum dots-based enzyme assays. We have developed a novel analytical assay to detect the lipase activity based on the enzyme-regulated quantum dots growth in situ. Catalytic hydrolytic cleavage of the ester bond in methyl thioglycolate (METG) will produce mercaptoacetate, and Na2S interacts with Cd(NO3)(2) to give CdS nanocrystals stabilized by mercaptoacetate. The fluorescence intensity changes, which depends on the absence or presence of lipase, could be used to sense the lipase activity. The detection limit (3 sigma) as low as 1.2 x 10(-2) mg mL(-1) with a linear range from 0.05 to 1.6 mg mL(-1) was achieved. A preliminary enzyme activity screening was carried out for four commercial purchased lipase samples, and the results are consistent with ones obtained from titration assay. Moreover, we applied this method to detecting lipase activity in fermentation broth of Bacillus subtilis without any pretreatment. The reported methodology shows potential applications for the development of a simple and inexpensive assay for the hydrolysis enzymes.
机译：脂肪酶是基于量子点的酶测定领域的被忽略的酶。我们已经开发了一种新的分析测定，以检测基于酶调节的量子点生长的脂肪酶活性。在巯基乙酸甲酯（METG）酯键的催化水解裂解会产生巯基乙酸酯，和硫化钠随着Cd（NO3）（2）相互作用，得到的CdS由巯基乙酸酯稳定的纳米晶体。可以使用荧光强度变化，这取决于脂肪酶的不存在或存在，可用于感测脂肪酶活性。实现了低至1.2×10（-2）mg ml（-1）的检测限（3 sigma），达到0.05至1.6mg ml（-1）的线性范围。对四种商业购买的脂肪酶样品进行初步酶活性筛选，结果与从滴定测定中获得的结果一致。此外，我们将这种方法应用于检测枯草芽孢杆菌发酵液中的脂肪酶活性而无需任何预处理。报告的方法显示了用于开发用于水解酶的简单且廉价的测定的潜在应用。

著录项

  • 来源
    《RSC Advances》 |2015年第89期|共7页
  • 作者

    Zhang Wei; Liu Jia; Zhang Lei; Gan Jian-Hong; Ding Yaqi; Huang Wei; Huo Feng-Wei; Tian Danbi;

  • 作者单位

    Nanjing Tech Univ Coll Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Coll Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Univ Posts &

    Telecommun Key Lab Organ Elect &

    Informat Displays Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Tech Univ Coll Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Coll Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Nanjing Tech KLOFE Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Nanjing Tech KLOFE Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Coll Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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