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首页> 外文期刊>RSC Advances >Chondrocytes behaviors within type I collagen microspheres and bulk hydrogels: an in vitro study
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Chondrocytes behaviors within type I collagen microspheres and bulk hydrogels: an in vitro study

机译：I型胶原蛋白微球和散装水凝胶中的软骨细胞行为：体外研究

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摘要

Cell niche, which is considered to be critical to the proliferation and differentiation of cells, is one of the most important aspects for the design and development of ideal scaffolds in tissue engineering. The mass transfer property of the scaffold affects the nutrient supply and exchange of the other substances. In this study, we prepared collagen hydrogels in the form of microspheres (CHMs) and bulk (CHB) to investigate the mass exchange differences and their influence on embedded chondrocytes. CHMs were developed by the emulsion method, which was efficient to load cells. Bovine serum albumin (BSA) was used as a diffusion model in the CHMs and CHB to evaluate the transport property of the hydrogels and the release kinetics. During the 4 week in vitro culture process, the contraction of the hydrogels, the cell viability and morphology, and the DNA and glycosaminoglycan (GAG) content were monitored at different intervals. The results suggested that the CHMs showed an obvious superiority in the transfer property over the CHB, leading to better maintenance of the chondrocyte phenotype in CHMs at the early stage of the in vitro culture. Histological analyses indicated that lots of lacunae and homogeneous positive GAG staining appeared in the CHMs from day 7. In contrast, only a few lacunae and obscure GAG staining were found in the outer area of the CHB after day 21. Without enough nutrients, the chondrocytes in the inner area of the CHB had little secreted matrix. Based on the presented CHM system, a further developed construct is suggested as a promising alternative toward the clinical application of engineered cartilaginous tissue.
机译：细胞Niche被认为对细胞的增殖和分化至关重要，是组织工程中理想支架设计和发展的最重要方面之一。支架的传质性能影响其他物质的营养供应和交换。在这项研究中，我们以微球（CHM）和散装（CHB）的形式制备胶原水凝胶，以研究大规模交换差异及其对嵌入的软骨细胞的影响。通过乳液法开发CHM，其有效地称为载体。牛血清白蛋白（BSA）用作CHM和CHB中的扩散模型，以评估水凝胶的运输性和释放动力学。在4周的体外培养过程中，用不同间隔监测水凝胶，细胞活力和形态的收缩，细胞活力和形态，以及DNA和糖胺聚糖（GAG）含量。结果表明，CHM在CHB上的转移特性显示出明显的优势，从而更好地维持体外培养早期CHMS中的软骨细胞表型。组织学分析表明，在第7天的CHM中出现了大量的空隙和均匀的阳性GAG染色。相反，在第21天之后只有几个裂变物和模糊的Gag染色。没有足够的营养，软骨细胞没有足够的营养在CHB的内部区域有很少的分泌基质。基于所呈现的CHM系统，建议进一步发育的构建体作为对工程软骨组织的临床应用的有前途的替代方案。

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  • 来源
    《RSC Advances》 |2015年第67期|共8页
  • 作者

    Liu Jun; Lin Hai; Li Xiupeng; Fan Yujiang; Zhang Xingdong;

  • 作者单位

    Sichuan Univ Natl Engn Res Ctr Biomat Chengdu 610064 Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat Chengdu 610064 Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat Chengdu 610064 Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat Chengdu 610064 Peoples R China;

    Sichuan Univ Natl Engn Res Ctr Biomat Chengdu 610064 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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