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A quartz crystal microbalance as a tool for biomolecular interaction studies

机译：一种石英晶体微稳定作为生物分子相互作用研究的工具

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摘要

A quartz crystal microbalance (QCM) and surface plasma resonance (SPR) are two typical solid-phase based technologies for biomolecular interaction studies. Although QCM is more affordable than SPR, the popularization of QCM as a biosensor is limited by the nonideal behavior that complicates the quantitative interpretation of frequency changes, especially the viscoelastic factor, implying that caution should be taken in interpreting QCM responses for large molecular applications. A poly(ethylene glycol) based matrix (PEG matrix) was tested on both QCM and SPR using model bait-prey pairs. Based on the dissipation monitoring technique, this matrix was found to have minimal viscoelasticity change before and after biomolecular binding. Furthermore, impedance analysis of frequency responses at multiple overtones was able to remove the viscoelastic contribution based on experimental results. Therefore, it is proved that the PEG matrix combined with the equations for impedance analysis of frequency changes at multiple overtones will facilitate the popularization of QCM as a biosensor.
机译：石英晶体微稳定（QCM）和表面等离子体共振（SPR）是两种用于生物分子相互作用研究的基于典型的固相技术。虽然QCM比SPR更实惠，但QCM作为生物传感器的普及受到非膜行为的限制，使得对频率变化的定量解释，尤其是粘弹性因子，这意味着应在解释大量分子应用的QCM反应中进行小心。使用模型诱饵对在QCM和SPR上测试聚（乙二醇）基质（PEG基质）。基于耗散监测技术，发现该基质在生物分子结合前后具有最小的粘弹性变化。此外，在多个ovoTones的频率响应的阻抗分析能够基于实验结果去除粘弹性贡献。因此，证明了PEG矩阵与多个overtoneS的频率变化的阻抗分析方程组合将促进QCM作为生物传感器的普及。

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  • 来源
    《RSC Advances》 |2015年第79期|共6页
  • 作者

    Wu Yuanzi; Ma Hongwei; Gu Dayong; He Jianan;

  • 作者单位

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion Suzhou 215125 Peoples R China;

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion Suzhou 215125 Peoples R China;

    Shenzhen Entry Exit Inspect &

    Quarantine Bur Shenzhen Int Travel Hlth Care Ctr Inst Dis Control &

    Prevent Shenzhen 518033 Peoples R China;

    Shenzhen Entry Exit Inspect &

    Quarantine Bur Shenzhen Int Travel Hlth Care Ctr Inst Dis Control &

    Prevent Shenzhen 518033 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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